1株梅花鹿源多重耐药大肠杆菌全基因组分析  

Whole-genome Sequencing of a Multidrug-resistant Strain of Escherichia coli Derived from Sika Deer

作　　者：周航[1] 杨海旺 孙宏飞 霍亚迪 孙婉莹 薛原[1] ZHOU Hang;YANG Haiwang;SUN Hongfei;HUO Yadi;SUN Wanying;XUE Yuan(College of Wildlife and Protected Area,Northeast Forestry University,Harbin Heilongjiang 150040,China)

机构地区：[1]东北林业大学野生动物与自然保护地学院,黑龙江哈尔滨150040

出　　处：《现代畜牧科技》2024年第6期43-46,共4页Modern Animal Husbandry Science & Technology

基　　金：黑龙江省博士后科研启动金(LBH-Q14002)。

摘　　要：该试验通过对药敏试验中具有多重耐药性的1株编号为64的梅花鹿源大肠杆菌进行全基因组测序,利用GO、KEGG、VFDB、CARD等数据库对菌株进行基因功能注释;利用Circos软件绘制基因组环形图谱。测序结果显示,该菌株的基因总长度4838417 bp,GC含量50.78%,含1个质粒,长度93378 bp。预测该菌株编码基因数量4514个,总长度4268565 bp,预测出该菌株含有14个基因岛。基因功能分析显示,该菌株基因组有3208条基因与KEGG数据库相对应,其中2009个基因参与到了49条信号通路。通过数据库比对显示该菌株基因组中存在61个耐药基因,对16类抗菌药物有抗性,存在898个毒力基因。该试验结果为大肠杆菌耐药性的研究及防控提供了一定的数据参考。This experiment sequenced the entire genome of a strain of Escherichia coli from Sika deer with multiple drug resistance in the drug sensitivity test,and annotated the gene function of the strain using databases such as GO,KEGG,VFDB,CARD,etc.Draw a genome circular map using Circos software.The sequencing results showed that the total gene length of the strain was 4838417 bp,with a GC content of 50.78%.Contains a plasmid with a length of 93378 bp.The predicted number of coding genes for this strain is 4514,with a total length of 4268565 bp.It is predicted that this strain contains 14 gene islands.Gene functional analysis shows that the genome of this strain contains 3208 genes corresponding to the KEGG database,of which 2009 genes are involved in 49 signaling pathways.Through database comparison,it was found that there are 61 resistance genes in the genome of this strain,which are resistant to 16 types of antibiotics,and 898 virulence genes.The results of this experiment provide certain data reference for the research and prevention and control of antibiotic resistance in Escherichia coli.

关 键 词：大肠杆菌 耐药性 全基因组测序 

分 类 号：S858.25[农业科学—临床兽医学]