葡萄糖/木糖混合发酵产乳酸过程调控研究  

Regulation of mixed glucose/xylose fermentation for l-lactic acid production

作　　者：孙轶男 李源 王娟 刘建国[1] SUN Yinan;LI Yuan;WANG Juan;LIU Jianguo(School of Resources and Environmental Engineering,Inner Mongolia University of Technology,Hohhot 010051,China;School of Civil Engineering,Inner Mongolia University of Technology,Hohhot 010051,China)

机构地区：[1]内蒙古工业大学资源与环境工程学院,呼和浩特010051 [2]内蒙古工业大学土木工程学院,呼和浩特010051

出　　处：《内蒙古工业大学学报（自然科学版）》2024年第3期219-223,共5页Journal of Inner Mongolia University of Technology：Natural Science Edition

基　　金：内蒙古自治区自然科学基金项目(2019LH05018);内蒙古工业大学科学研究项目(ZZ201808)。

摘　　要：木质纤维素水解后会形成以葡萄糖和木糖为主的混合液,而乳酸菌在利用这种混糖发酵生产乳酸的过程中由于碳分解代谢阻遏效应的原因使得乳酸产量较低。本研究通过调节混糖比以及添加碳酸钙、维生素B、稀土元素等方法提高混糖发酵的乳酸产量。结果表明,当总还原糖浓度为50 g/L,混糖比为葡萄糖∶木糖=4∶1,初始pH为9.0,发酵温度为37℃时,乳酸产量最高;当分别添加30 g/L的碳酸钙、5 g/L的维生素B、5 mg/L的氯化钕、30 mg/L的氯化铒时,乳酸浓度分别提高了27.07%、25.4%、23.10%和23.14%。本研究通过对混糖发酵体系的优化为木质纤维素类物质发酵生产乳酸提供了理论基础。After the hydrolysis of lignocellulosic cellulose,a mixed solution mainly composed of glucose and xylose is formed.However,during the fermentation process of lactobacillus utilizing this mixed sugar to produce lactic acid,the carbon degradation metabolism inhibition effect leads to a lower lactic acid yield.This study aimed to increase the lactic acid production during mixed sugar fermentation by adjusting the sugar ratio and adding calcium carbonate,vitamin B,rare earth elements,and other methods.The results showed that the highest lactic acid production was achieved when the total reducing sugar concentration was 50 g/L,the sugar ratio was glucose/xylose=4:1,the initial pH was 9.0,and the fermentation temperature was 37℃.Additionally,the addition of 30 g/L calcium carbonate,5 g/L vitamin B,5 mg/L neodymium chloride,and 30 mg/L erbium chloride increased the lactic acid concentration by 27.07%,25.4%,23.10%,and 23.14%respectively.This study provides a theoretical basis for the fermentation of lignocellulosic materials to produce lactic acid through the optimization of the mixed sugar fermentation system.

关 键 词：植物乳杆菌 乳酸 混糖发酵 维生素B 稀土元素 

分 类 号：Q815[生物学—生物工程]