正交试验优选千金子制霜工艺及制霜前后脂肪油成分变化分析  

Optimization of Frosting Process of Semen Euphorbiae with Orthogonal Design and Comparison Analysis of Fatty Oil Components Before and After Frosting

作　　者：魏晓彤 石双慧 王梦琳 马思媛 胡宇峰 姜明瑞 张婧秋 王慧楠 梁少国 王英姿[1] Wei Xiaotong;Shi Shuanghui;Wang Menglin;Ma Siyuan;Hu Yufeng;Jiang Mingrui;Zhang Jingqiu;Wang Huinan;Liang Shaoguo;Wang Yingzi(Beijing University of Chinese Medicine,Beijing 102488,China)

机构地区：[1]北京中医药大学,北京102488

出　　处：《中国药事》2024年第5期567-574,共8页Chinese Pharmaceutical Affairs

基　　金：国家自然科学基金资助项目(编号82374040)。

摘　　要：目的:优选得到千金子最佳机械压榨制霜工艺,明确其工艺参数,并分析制霜前后千金子脂肪油成分的变化情况。方法:以2020年版《中华人民共和国药典》中规定的千金子霜外观性状和脂肪油含量作为评价指标,采用L9(34)正交试验法优选千金子机械压榨制霜工艺的制霜温度、压制时间和制霜压力,以确定千金子最佳机械压榨制霜工艺;采用气相色谱-质谱联用(GC-MS)技术对千金子制霜前后脂肪油成分进行分析。结果:优选得到最佳机械压榨制霜工艺条件:取千金子种仁碾成泥状(粒度约为40~60目),在50℃、50 MPa的压力下压制1次,压制时间为10 min。千金子生品和霜品脂肪油中共检出14种共有脂肪酸甲酯,制霜前后脂肪油中脂肪酸成分组成基本一致,相对含量略有差别,但变化不大。结论:优选得到的千金子机械压榨制霜工艺稳定可靠,简便易行,可为千金子霜饮片生产和质量控制提供参考。Objective:To optimize the best mechanical compression frosting process of Semen Euphorbiae,clarify its process parameters,and analyze the changes of fatty oil of Semen Euphorbiae before and after frosting.Methods:The appearance characteristics and fatty oil content of Semen Euphorbiae Pulveratum in the 2020 edition of Pharmacopoeia of the People's Republic of China were used as evaluation indexes.The L9(34)orthogonal test method was used to optimize the frosting temperature,compression time and frosting pressure of the mechanical compression frosting process of Semen Euphorbiae to determine the best mechanical compression frosting process of Semen Euphorbiae.The fatty oils before and after frosting were analyzed by gas chromatography-mass spectrometry(GC-MS).Results:The optimum mechanical compression frosting process conditions were as follows: the Semen Euphorbiae seed kernels were ground into mud (particle size of about 40-60 mesh), pressed once at 50 °C and 50 MPa pressure for 10 min. A total of 14 common fatty acid methyl esterswere detected in the fatty oil of raw and frosted products of Semen Euphorbiae. The composition of fatty acidcomponents in the fatty oil before and after frosting was basically the same, with slight diff erences in relativecontent, but little changes. Conclusion: The optimized mechanical compression frosting process of SemenEuphorbiae is stable, reliable and simple, which can provide reference for the production and quality control ofSemen Euphorbiae Pulveratum decoction pieces.

关 键 词：千金子 炮制工艺 正交试验 脂肪油 气相色谱-质谱联用 

分 类 号：R95[医药卫生—药学] R932