Growth hormone improves insulin resistance in visceral adipose tissue after duodenal-jejunal bypass by regulating adiponectin secretion  

作　　者：Zi-Tian Liu Guang-Wei Yang Xiang Zhao Shuo-Hui Dong Yang Jiao Zheng Ge Ao Yu Xi-Qiang Zhang Xin-Zhen Xu Zhi-Qiang Cheng Xiang Zhang Ke-Xin Wang 

机构地区：[1]Department of General Surgery,Qilu Hospital of Shandong University,Jinan 250012,Shandong Province,China [2]Department of General Surgery,Shandong University of Qilu Hospital(Qingdao),Qingdao 266000,Shandong Province,China

出　　处：《World Journal of Diabetes》2024年第6期1340-1352,共13页世界糖尿病杂志（英文版）（电子版）

基　　金：Supported by National Natural Science Foundation of China(General Program),No.82070852 and No.82270901.

摘　　要：BACKGROUND The mechanism of improvement of type 2 diabetes after duodenal-jejunal bypass(DJB)surgery is not clear.AIM To study the morphological and functional changes in adipose tissue after DJB and explore the potential mechanisms contributing to postoperative insulin sensitivity improvement of adipose tissue in a diabetic male rat model.METHODS DJB and sham surgery was performed in a-high-fat-diet/streptozotocin-induced diabetic rat model.All adipose tissue was weighed and observed under microscope.Use inguinal fat to represent subcutaneous adipose tissue(SAT)and mesangial fat to represent visceral adipose tissue.RNA-sequencing was utilized to evaluate gene expression alterations adipocytes.The hematoxylin and eosin staining,reverse transcription-quantitative polymerase chain reaction,western blot,and enzyme-linked immunosorbent assay were used to study the changes.Insulin resistance was evaluated by immunofluorescence.RESULTS After DJB,whole body blood glucose metabolism and insulin sensitivity in adipose tissue improved.Fat cell volume in both visceral adipose tissue(VAT)and SAT increased.Compared to SAT,VAT showed more significantly functional alterations after DJB and KEGG analysis indicated growth hormone(GH)pathway and downstream adiponectin secretion were involved in metabolic regulation.The circulating GH and adiponectin levels and GH receptor and adiponectin levels in VAT increased.Cytological experiment showed that GH stimulated adiponectin secretion and improve insulin sensitivity.CONCLUSION GH improves insulin resistance in VAT in male diabetic rats after receiving DJB,possibly by increasing adiponectin secretion.

关 键 词：Growth hormone Insulin resistance Bariatric surgery Adipose tissue ADIPONECTIN 

分 类 号：R711.75[医药卫生—妇产科学] R589[医药卫生—临床医学]