Diagnostic and prognostic role of LINC01767 in hepatocellular carcinoma  

作　　者：Li Zhang Tong-Xing Cui Xiang-Zhi Li Chong Liu Wen-Qin Wang 

机构地区：[1]Department of Thyroid and Breast Surgery,The Affiliated People Hospital of Second Medical University,Weifang 266010,Shandong Province,China [2]Department of General Surgery,Qingdao Municipal Hospital Group,Qingdao 266237,Shandong Province,China [3]School of Life Sciences,Shandong University(Qingdao),Qingdao 26637,Shandong Province,China [4]School of Medicine,Tsinghua University,Beijing 100084,China

出　　处：《World Journal of Hepatology》2024年第6期932-950,共19页世界肝病学杂志（英文版）（电子版）

基　　金：Supported by Foundation of Qingdao Postdoctoral Innovation Project,No.QDBSH20230101019;Funded State Key Laboratory of Marine Food Processing&Safety Control,Qingdao,No.SKL2023M05.

摘　　要：BACKGROUND Hepatocellular carcinoma(HCC)is a primary contributor to cancer-related mortality on a global scale.However,the underlying molecular mechanisms are still poorly understood.Long noncoding RNAs are emerging markers for HCC diagnosis,prognosis,and therapeutic target.No study of LINC01767 in HCC was published.AIM To conduct a multi-omics analysis to explore the roles of LINC01767 in HCC for the first time.METHODS DESeq2 Package was used to analyze different gene expressions.Receiver operating characteristic curves assessed the diagnostic performance.Kaplan-Meier univariate and Cox multivariate analyses were used to perform survival analysis.The least absolute shrinkage and selection operator(LASSO)-Cox was used to identify the prediction model.Subsequent to the validation of LINC01767 expression in HCC fresh frozen tissues through quantitative real time polymerase chain reaction,next generation sequencing was performed following LINC01767 over expression(GSE243371),and Gene Ontology/Kyoto Encyclopedia of Genes and Genomes/Gene Set Enrichment Analysis/ingenuity pathway analysis was carried out.In vitro experiment in Huh7 cell was carried out.RESULTS LINC01767 was down-regulated in HCC with a log fold change=1.575 and was positively correlated with the cancer stemness.LINC01767 was a good diagnostic marker with area under the curve(AUC)[0.801,95% confidence interval(CI):0.751-0.852,P=0.0106]and an independent predictor for overall survival(OS)with hazard ratio=1.899(95%CI:1.01-3.58,P=0.048).LINC01767 nomogram model showed a satisfied performance.The top-ranked regulatory network analysis of LINC01767 showed the regulation of genes participating various pathways.LASSO regression identified the 9-genes model showing a more satisfied performance than 5-genes model to predict the OS with AUC>0.75.LINC01767 was down-expressed obviously in tumor than para-tumor tissues in our cohort as well as in cancer cell line;the over expression of LINC01767 inhibit cell proliferation and clone formation of Huh7 in vitro.CONCL

关 键 词：Hepatocellular carcinoma LINC01767 Multi-omics analysis GSE243371 Cell proliferation Clone formation 

分 类 号：R735.7[医药卫生—肿瘤]