乳腺癌组织ALEX1水平与临床特征相关性分析  

Analysis of correlation between ALEX1 level and clinical features in breast cancer tissues

作　　者：刘启榕[1] 伍毓强[1] 乐桂花[1] 邓晓玲 罗媛 LIU Qirong;WU Yuqiang;YUE Guihua;DENG Xiaoling;LUO Yuan(The Second Hospital of Sanming,Sanming 366000,China)

机构地区：[1]三明市第二医院,福建三明366000

出　　处：《包头医学院学报》2024年第6期61-65,96,共6页Journal of Baotou Medical College

摘　　要：目的:探讨免疫组化检测乳腺癌组织病理ALEX1水平与临床特征的相关性。方法:77例乳腺癌及癌旁2 cm标本分别采用免疫组化、免疫印迹及PCR检测ALEX1阳性率、蛋白及mRNA水平,并进行与临床病理特征的相关性分析。结果:ALEX1在77例乳腺癌组织及癌旁组织细胞膜上呈现黄色及黄棕色表达,ALEX1在乳腺癌组织的阳性表达有45例(58.44%),在癌旁组织有61例,阳性率为79.22%,组间比较有明显差异(χ^(2)=4.199,P<0.05);ALEX1在77例乳腺癌组织及癌旁组织蛋白表达分别为2.05±0.55、4.11±0.69,ALEX1在乳腺癌组织中蛋白含量低于癌旁组织,组间比较存在显著差异(t=20.85,P<0.001);ALEX1 mRNA在77例癌旁组织及乳腺癌组织中表达为1.00±0.00、0.45±0.08,ALEX1mRNA在不同组织中表达存在明显差异(t=60.330,P<0.001);ALEX1表达与病理类型、病理等级和淋巴结转移存在相关性(P<0.05),与其他指标均无相关性(P>0.05);按照3分为界限,观察ALEX1水平高低与临床病理关系,ALEX1水平降低与乳腺癌患者浸润性导管癌、病理等级Ⅱ~Ⅲ具有相关性(P<0.05);ALEX1在乳腺癌疾病中AUC值为0.646,特异性及灵敏度分别为55.42%、65.02%,说明检测ALEX1表达水平对乳腺癌病理诊断存在意义。结论:ALEX1在癌旁组织中表达水平显著高于乳腺癌组织,ALEX1表达降低与乳腺癌淋巴结转移及病理分级升高相关。Objective:To explore the correlation between ALEX1 level and clinical features of breast cancer histopathologic detection by immunohistochemistry.Methods:ALEX1 positive rate,protein and mRNA levels were detected by immunohistochemistry,western blotting and PCR in 77 cases of breast cancer and 2 cm adjacent specimens,and the correlation between ALEX1 and clinicopathologic features was analyzed.Results:ALEX1 showed yellow and yellowish brown expression in the cell membrane of 77 breast cancer tissues and adjacent tissues.ALEX1 positive expression in 45 breast cancer tissues was 58.44%,and positive rate in 61 adjacent tissues was 79.22%.There was significant difference between groups(χ^(2)=4.199,P<0.05).The protein expression of ALEX1 in 77 breast cancer tissues and adjacent tissues was 2.05±0.55 and 4.11±0.69,respectively.The protein content of ALEX1 in breast cancer tissues was lower than that in adjacent tissues,and there was significant difference between groups(t=20.85,P<0.001).The expression of ALEX1mRNA was 1.00±0.00 and 0.45±0.08 in 77 paracancer tissues and breast tissues,and there were significant differences in the expression of ALEX1mRNA in different tissues(t=60.330,P<0.001).ALEX1 expression was correlated with pathological type,pathological grade and lymph node metastasis(P<0.05),but not with other indicators(P>0.05).The relationship between ALEX1 level and clinicopathology was observed according to the 3 classification boundaries.The decreased level of ALEX1 was correlated with invasive ductal carcinoma and pathological gradeⅡtoⅢof breast cancer patients(P<0.05).The AUC area of ALEX1 in breast cancer was 0.646,and the specificity and sensitivity were 55.42%and 65.02%,respectively,indicating the significance of ALEX1 expression detection in the pathological diagnosis of breast cancer.Conclusion:The expression level of ALEX1 in paracancer tissues is significantly higher than that in breast cancer tissues,and the decreased expression of ALEX1 is associated with increased lymph node metastasis an

关 键 词：ALEX1 乳腺癌 临床病理 免疫组化 ROC曲线分析 

分 类 号：R737.9[医药卫生—肿瘤]