皮肤鳞状细胞癌组织微RNA-103a-2-5p、钙黏附蛋白11表达及其临床意义  

Expression and clinical significance of miR-103a-2-5p and CDH11 in cutaneous squamous cell carcinoma

作　　者：赵云超 宋颖[1] ZHAO Yunchao;SONG Ying(Department of Dermatology,Baoji People´s Hospital,Baoji,Shaanxi 721000,China)

机构地区：[1]宝鸡市人民医院皮肤科,陕西宝鸡721000

出　　处：《安徽医药》2024年第7期1372-1377,共6页Anhui Medical and Pharmaceutical Journal

基　　金：陕西省卫生健康科研项目(2022D015)。

摘　　要：目的分析皮肤鳞状细胞癌组织中微RNA-103a-2-5p(miR-103a-2-5p)和钙黏附蛋白11(CDH11)的表达水平,探讨二者在临床研究中的意义。方法选取2017年1月至2019年6月宝鸡市人民医院诊治的98例皮肤鳞状细胞癌病人为研究对象,收集病人术中切除的癌组织及癌旁正常组织。采用实时荧光定量PCR(qRT-PCR)法检测组织中miR-103a-2-5p的表达水平。采用酶联免疫吸附(ELISA)法检测CDH11表达水平。TargetScanHuman网站预测miR-103a-2-5p与CDH11的靶向关系;Pearson相关性分析癌组织中miR-103a-2-5p和CDH11水平的关系;Kaplan-Meier生存曲线分析miR-103a-2-5p、CDH11表达与皮肤鳞状细胞癌病人预后的关系;影响皮肤鳞状细胞癌病人预后的因素采用Cox回归分析;受试者操作特征曲线(ROC曲线)分析miR-103a-2-5p和CDH11的表达对皮肤鳞状细胞癌的诊断价值。结果与癌旁正常组织[1.03±0.12,(5.06±1.43)µg/L]相比,皮肤鳞状细胞癌组织miR-103a-2-5p表达水平(1.46±0.38)显著升高(P<0.05),CDH11表达水平[(2.96±0.62)µg/L]明显降低(P<0.05);TargetScanHu-man网址预测miR-103a-2-5p与CDH11间存在结合位点;经Pearson相关性分析,miR-103a-2-5p与CDH11水平呈负相关(P<0.05);两者均与病人病理分级、TNM分期、淋巴结转移、侵袭程度相关(P<0.05);miR-103a-2-5p高表达组和CDH11低表达组复发率显著高于miR-103a-2-5p低表达组和CDH11高表达组(P<0.05);TNM分期、淋巴结转移、miR-103a-2-5p是影响病人预后的危险因素(P<0.05),CDH11是影响病人预后的保护因素(P<0.05);miR-103a-2-5p、CDH11二者联合诊断皮肤鳞状细胞癌的ROC曲线下面积(AUC)为0.836,显著优于其各自单独诊断(P=0.018、0.021)。结论皮肤鳞状细胞癌病人miR-103a-2-5p表达水平升高,CDH11表达水平降低,二者均与病人临床病理特征及预后有密切关系,且二者联合可较好的诊断皮肤鳞状细胞癌。Objective To analyze the expression levels of microRNA-103a-2-5p(miR-103a-2-5p)and cadherin 11(CDH11)in skin squamous cell carcinoma(SCC),and to explore their clinical significance.Methods Ninety-eight patients with skin squamous cell carcinoma diagnosed and treated in Baoji People´s Hospital from January 2017 to June 2019 were selected as the study objects,and the cancerous tissues and normal tissues adjacent to the cancer were collected.The miR-103a-2-5p level in tissues was detected by realtime fluorescent quantitative PCR(qRT-PCR).The expression of CDH11 was detected by enzyme-linked immunosorbent assay(ELISA).TargetScanHuman website predicted the target relationship between miR-103a-2-5p and CDH11.Pearson correlation analysis was performed on the expression levels of miR-103a-2-5p and CDH11 in cancer tissues.Kaplan-Meier survival curve was used to analyze the relationship between miR-103a-2-5p,CDH11 expressions and prognosis of patients with cutaneous squamous cell carcinoma(CSCC);the factors affecting prognosis of patient with SCC were analyzed using Cox regression analysis;the diagnostic value of miR-103a-2-5p and CDH11 expressions in CSCC was analyzed by ROC curve.Results Compared with normal tissue adjacent to cancer[miR-103a-2-5p:1.03±0.12,CDH11:(5.06±1.43)μg/L],the expression level(1.46±0.38)of miR-103a-2-5p in skin SCC tissue was significantly increased(P<0.05),while the expression level[(2.96±0.62)μg/L]of CDH11 was lower(P<0.05).TargetScanHuman website predicted that there was a binding site between miR-103a-2-5p and CDH11.There was a negative correlation between miR-103a-2-5p and CDH11 expression(P<0.05),both of which were correlated with pathological grade,TNM stage,lymph node metastasis and invasion degree(P<0.05).The recurrence rate was significantly higher in the miR-103a-2-5p high-expression group and the CDH11 low-expression group than in the miR-103a-2-5p low-expression group and the CDH11 high-expression group(P<0.05);TNM stage,lymph node metastasis,and miR-103a-2-5p were risk factors

关 键 词：皮肤鳞状细胞癌 微RNA-103a-2-5p 钙黏附蛋白11 癌组织 预后 

分 类 号：R739.5[医药卫生—肿瘤]