补骨脂种子提取物对茶饼病菌的抑菌活性  

The antifungal activity of Psoralea corylifolia seeds extract against Exobasidium vexans

作　　者：孙礼雪 关丽杰 马晓彤 高洁 张洪雨 商今雨 SUN Li-xue;GUAN Li-jie;MA Xiao-tong;GAO Jie;ZHANG Hong-yu;SHANG Jin-yu(School of Pharmaceutical and Bioengineering,Shenyang University of Chemical Technology,Shenyang 110142,China;Chaoyang Yanwangbizi Reservoir Construction Administration,Chaoyang 122099,Liaoning,China)

机构地区：[1]沈阳化工大学制药与生物工程学院,沈阳110142 [2]朝阳市阎王鼻子水库工程建设管理局,辽宁朝阳122099

出　　处：《湖北农业科学》2024年第5期50-55,共6页Hubei Agricultural Sciences

基　　金：国家重点研发计划项目(SQ2020YFF0411744);辽宁省教育厅项目(LJKMZ20220787)。

摘　　要：探讨补骨脂(Psoralea corylifolia L.)种子提取物对茶饼病菌(Exobasidium vexans Massee)的抑菌活性及田间防治效果。采用孢子萌发抑制率法和菌丝生长速率法测定补骨脂种子提取物的抑菌活性,按照农药田间药效试验准则在5省5地进行茶饼病田间试验。室内抑菌活性试验结果表明,补骨脂种子提取物对茶饼病菌孢子萌发和菌丝生长具有明显的抑制作用,抑制率随着药剂浓度的增加而增加,其EC50分别为12.925 mg/L和6.074 mg/L。田间试验结果表明,当补骨脂种子提取物微乳剂有效成分用量为2.00~2.67 mg/kg时,其对茶饼病的防治效果为68.08%~83.78%,其防治效果相当或优于对照药剂3%多抗霉素可湿性粉剂。The antifungal activity and the field control effects of Psoralea corylifolia seeds extract against Exobasidium vexans were ex-plored.The spore germination inhibition rate method and mycelium growth rate method were used to determine the antifungal activity of Psoralea corylifolia seeds extract,and the field trials of Exobasidium vexans were carried out in five provinces and five places ac-cording to the pesticide field efficacy trials guideline.The indoor antifungal activity test showed that Psoralea corylifolia seeds extract had an obvious inhibitory effect on the spore germination and mycelium growth of Exobasidium vexans,and the inhibition rate in-creased with the increase of drug concentration,with the EC50 of 12.925 mg/L and 6.074 mg/L,respectively.The results of field trials showed that when the dosage of active ingredients of Psoralea corylifolia seeds extract microemulsion was 2.00~2.67 mg/kg,the field control effects of Exobasidium vexans was 68.08%~83.78%,which was equivalent to or better than that of the control agent 3%polyox-in wettable powder.

关 键 词：生物农药 茶饼病菌(Exobasidium vexans Massee) 抑菌活性 田间防治效果 补骨脂(Psoralea corylifolia L.)种子提取物 

分 类 号：S432[农业科学—植物病理学] S435.711[农业科学—农业昆虫与害虫防治]