间充质干细胞外泌体对缺血性脑卒中大鼠神经功能恢复的影响  

Effect of mesenchymal stem cell exosomes to neurological function recovery of rats with ischemic stroke

作　　者：刘君鹏[1] 李云飞[1] 李永坤[1] LIU Junpeng;LI Yunfei;LI Yongkun(Department of Neurology,Provincial Clinical Medical College of Fujian Medical University,Fujian Provincial Hospital,Fuzhou University Affiliated Provincial Hospital,Fujian Province,Fuzhou 350001,China)

机构地区：[1]福建医科大学省立临床医学院,福建省立医院,福州大学附属省立医院神经内科,福建福州350001

出　　处：《中国当代医药》2024年第17期4-8,共5页China Modern Medicine

基　　金：福建省自然科学基金资助项目(2020J011093)。

摘　　要：目的探讨间充质干细胞外泌体(MSCs-EXO)对缺血性脑卒中大鼠神经功能恢复的影响。方法大鼠骨髓间充质干细胞原代培养,超速离心法提取其MSCs-EXO,大脑中动脉夹闭模型(MCAO)法制作大鼠缺血性脑卒中模型,MSCs-EXO经鼻给药,设为MSCs-EXO组,通过改良神经功能缺损评分(mNSS)和错步试验评估其神经功能恢复情况,并与未治疗模型组(MCAO组)做对比。PKH26标记MSCs-EXO并结合免疫荧光染色观察其在缺血半暗带(IP)中的分布,荧光定量PCR及Western blot检测IP区域脑组织中PTEN的表达水平,并与正常大鼠及MCAO组大鼠进行对比。结果MSCs-EXO治疗组的神经功能恢复高于MCAO组,差异有统计学意义(P<0.05)。免疫荧光染色显示标记外泌体可以进入IP区域神经细胞,荧光定量PCR及Western blot检测显示MSCs-EXO治疗组及MCAO组中PTEN水平均较正常升高,但MSCs-EXO治疗组的PTEN水平低于MCAO组,差异有统计学意义(P<0.05)。结论MSCs-EXO能够促进缺血性脑卒中大鼠的神经功能恢复,这可能与其下调IP区域PTEN表达水平相关。Objective To discuss the effect of mesenchymal stem cell exosomes(MSCs-EXO)to neurological function recovery of rats with ischemic stroke.Methods Rat bone marrow mesenchymal stem cells were cultured in primary culture,MSCs-EXO were extracted by hyperspeed centrifuge method,and the rat ischemic stroke model was made by middle cerebral artery occlusion(MCAO)method,the MSCs-EXO were administered nasally and were set as MSCs-EXO treated group,and the neural function recovery was evaluated by modified neurological severity scores(mNSS)and misstep test,and compared with the untreated model group(MCAO group).The distribution of MSCs-EXO labeled by PKH26 in ischemic penumbra(IP)was observed by immunofluorescence staining.The expression level of PTEN in brain tissue in IP region was detected by fluorescence quantitative PCR and Western blot,and compared with normal rats and MCAO group.Results The recovery of nerve function in the MSCs-EXO treated group was higher than that in the MCAO group,and the difference was statistically significant(P<0.05).Immunofluorescence staining showed that labeled MSCs-EXO could enter nerve cells in the IP region.Quantitative fluorescent PCR and Western blot detection showed that PTEN levels in both the MSCs-EXO treated group and the MCAO group were higher than normal,but PTEN level in the MSCs-EXO treated group was lower than that in the MCAO group,with statistical significances(P<0.05).Conclusion MSCs-EXO can promote the recovery of neural function in ischemic stroke rats,which may be related to the down-regulation of PTEN expression in IP region.

关 键 词：间充质干细胞 外泌体 缺血性脑卒中 人第10号染色体缺失的磷酸酶及张力蛋白同源的基因 大鼠模型 

分 类 号：R743.3[医药卫生—神经病学与精神病学]