通辽部分地区鸭源大肠杆菌耐药性检测及多重耐药菌株YA-1全基因组测序分析  

Detection of Drug Resistance of Escherichia coli from Ducks in Some Areas of Tongliao and Whole Genome Sequencing Analysis of Multi-drug Resistant Strain YA-1

作　　者：王梓 孙淼 王永强 孟令浩 耿超 石金川 陈伟 邱军 刘锴[1,2] WANG Zi;SUN Miao;WANG Yongqiang;MENG Linghao;GENG Chao;SHI Jinchuan;CHEN Wei;QIU Jun;LIU Kai(College of Animal Science and Technology,Inner Mongolia Minzu University,Tongliao 028000,China;Inner Mongolia Engineering Technology Research Center for Prevention and Control of Beef Cattle Diseases,Tongliao 028000,China;Zhalantun Vocational College,Hulunbeier 162650,China;Inner Mongolia Autonomous Region Xilin Gol League East Ujimqin Banner Animal Husbandry Workstation,Xilin Gol 026399,China)

机构地区：[1]内蒙古民族大学动物科技学院,通辽028000 [2]内蒙古自治区肉牛疾病防控工程技术研究中心,通辽028000 [3]扎兰屯职业学院,呼伦贝尔162650 [4]内蒙古自治区锡林郭勒盟东乌珠穆沁旗畜牧工作站,锡林郭勒026399

出　　处：《中国畜牧兽医》2024年第6期2585-2596,共12页China Animal Husbandry & Veterinary Medicine

基　　金：内蒙古自治区自然科学基金(2021LHMS03005、2021BS03015);内蒙古民族大学博士启动基金(BS593、BS568);内蒙古自治区中央引导地方发展基金项目(2022ZY0149);高寒草原地区肉用西门塔尔牛绿色高效安全养殖技术集成研究与示范(2022YFDZ0013);内蒙古锡林郭勒盟科技计划项目(202213);内蒙古自治区高校基本科研业务费项目(GXKY22097)。

摘　　要：【目的】大肠杆菌作为家禽最常见细菌性病原之一,其耐药性的不断增强已引起广泛关注。试验旨在了解通辽地区部分鸭场大肠杆菌的耐药性及耐药基因分布,为该地区鸭源大肠杆菌耐药性及耐药基因的研究提供参考。【方法】经16S rRNA测序、生化试验和小鼠致病性试验对送检的通辽市各旗县15份病死鸭肠道样品进行病原菌分离纯化,对分离菌株进行药敏试验及耐药基因的PCR检测,并选取多重耐药菌株进行全基因组测序。【结果】所分离的10株鸭源大肠杆菌均具有不同程度耐药性,对链霉素、环丙沙星、恩诺沙星等药物的耐药率在80%及以上,耐药基因aphA1、strB和qnrS的检出率最高,达到100%。多重耐药菌株YA-1全基因组测序证实,该菌株染色体大小为4 844 827 bp,携带3个完整的质粒,大小分别为144 776 bp(pTLYA-1)、113 584 bp(pTLYA-2)和77 544 bp(pTLYA-3)。分离菌株携带arr-3、aadA16、sul1等70个耐药基因,其中pTLYA-1中携arr-3、dfrA27、aadA16等8个可移动耐药基因,pTLYA-3中携带floR、tetA、sul2等5个可移动耐药基因,pTLYA-2中未发现耐药基因。【结论】由通辽地区部分鸭场分离的鸭源大肠杆菌存在多重耐药性,耐药水平较高,aphA1、strB和qnrS耐药基因普遍流行。【Objective】As one of the most common bacterial pathogens in poultry,the increasing resistance of Escherichia coli(E.coli)has attracted wide attention.The purpose of this study was to understand the distribution of drug resistance and drug resistance genes of E.coli in some duck farms in Tongliao area,so as to provide reference for the study of drug resistance and drug resistance genes of E.coli from ducks in this area.【Method】The pathogenic bacteria were isolated and purified from 15 intestinal samples of dead ducks in each county of Tongliao city by 16S rRNA sequencing,biochemical test and mouse pathogenicity test.The isolated strains were subjected to drug sensitivity test and PCR detection of drug resistance genes,and multi-drug resistant strains were selected for whole genome sequencing.【Result】All 10 strains of E.coli had different degrees of drug resistance.The resistance rates to streptomycin,ciprofloxacin,ofloxacin and enrofloxacin were all above 80%,and the detection rates of drug resistance genes aphA 1,strB and qnrS were 100%.The whole genome sequencing of the multi-drug resistant strain YA-1 confirmed that the chromosome size of the strain was 4844827 bp,carrying 3 complete plasmids,which were 144776 bp(pTLYA-1),113584 bp(pTLYA-2)and 77544 bp(pTLYA-3),respectively.The isolated strains carried 70 drug resistance genes such as arr-3,aadA 16 and sul 1.Among them,pTLYA-1 carried 8 mobile drug resistance genes such as arr-3,dfrA 27 and aadA 16,pTLYA-3 carried 5 mobile drug resistance genes such as floR,tetA and sul 2,and no drug resistance genes were found in pTLYA-2.【Conclusion】E.coli isolated from some duck farms in Tongliao area had multiple drug resistance,and the resistance level was high.The resistance genes of aphA 1,strB and qnr S were prevalent.

关 键 词：鸭 大肠杆菌 耐药性 耐药基因 全基因组测序 

分 类 号：S852.617[农业科学—基础兽医学]