MicroRNA-26b下调MALAT-1抑制乳腺癌MCF-7细胞恶性生物学行为的机制研究  

MicroRNA-26b inhibits the malignant biological behavior of MCF-7 breast cancer cells by down-regulating MALAT-1

作　　者：阮思蓓 熊小明 赵梓亦[2] 杨青坤 张翠薇 Ruan Si-bei;Xiong Xiao-ming;Zhao Zi-yi;Yang Qing-kun;Zhang Cui-wei(Department of Pathology,The Affiliated Hospital of Southwest Medical University,Luzhou,Sichuan 646000,China;TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province,Hospital of Chengdu University of Traditional Chinese Medicine,Chengdu,Sichuan 610000,China)

机构地区：[1]西南医科大学附属医院病理科,四川泸州646000 [2]成都中医药大学附属医院中医药调节代谢性疾病四川省重点实验室,四川成都610000

出　　处：《中国现代医学杂志》2024年第12期17-23,共7页China Journal of Modern Medicine

基　　金：四川省科技厅重点项目(No:2022YFS0404);四川省中医药管理局2021年度中医药科研专项(No:2021MS510);泸州市人民政府-西南医科大学科技战略合作应用基础研究项目(No:2019LZXNYDJ13)。

摘　　要：目的 探索microRNA-26b(miR-26b)通过MALAT-1抑制乳腺癌MCF-7细胞恶性生物学行为的分子机制。方法 以乳腺癌细胞系MCF-7作为研究对象,采用慢病毒LV-miR-26b-ctrl和LV-miR-26b转染肿瘤细胞,逆转录聚合酶链反应检测MALAT-1、miR-26a和miR-26b的mRNA表达,平板克隆形成实验、CCK-8细胞增殖实验、软琼脂成瘤实验、细胞划痕实验、Transwell细胞侵袭实验分别检测肿瘤细胞的增殖、体外成瘤、迁移和侵袭能力。结果 E2组与Mock组MCF-7细胞24、48、72和96 h时吸光度值比较,经重复测量设计的方差分析,结果:(1)不同时间点吸光度值比较,差异有统计学意义(P <0.05);(2)两组吸光度值比较,差异有统计学意义(P <0.05),与Mock组比较,E2组72和96 h时细胞增殖能力较Mock组提高(P <0.05);(3)两组吸光度值变化趋势比较,差异有统计学意义(P <0.05)。与Mock组相比,E2组MALAT-1相对表达量升高(P <0.05),miR-26a、miR-26b mRNA相对表达量下降(P <0.05)。高表达miR-26b的乳腺癌患者的生存情况优于低表达miR-26b组,死亡风险更低(P <0.05),低表达miR-26a组患者的生存情况优于高表达miR-26a组(P <0.05),用Cox比例风险回归模型,将miR-26a作为一个独立的预后因素来比较,两组患者的死亡风险比较无差异(P>0.05)。与LV-miR-26b-ctrl组比较,LV-miR-26b-ctrl/E2组乳腺癌细胞的MALAT-1相对表达量升高(P <0.05),与LV-miR-26b-ctrl/E2组比较,LV-miR-26b/E2组乳腺癌细胞的MALAT-1相对表达量降低(P <0.05)。LV-miR-26b-ctrl组、LV-miR-26b-ctrl/E2组、LV-miR-26b/E2组细胞在24、48、72和96 h时吸光度值比较,经重复测量设计的方差分析,结果:(1)不同时间点吸光度值比较,差异有统计学意义(P <0.05);(2)各组吸光度值比较,差异有统计学意义(P <0.05),LV-miR-26b-ctrl/E2组72和96 h时细胞增殖能力明显较LV-miR-26b-ctrl组增强(P <0.05),LV-miR-26b/E2组72和96 h时细胞增殖能力较LV-miR-26b-ctrl/E2组降低(P <0.05);(3)各组吸光度值变化趋势�Objective To explore the molecular mechanism of microRNA-26b(miR-26b)inhibiting the malignant biological behavior of MCF-7 breast cancer cells through MALAT-1.Methods MCF-7 breast cancer cell line was transduced with lentivirus-based vectors LV-miR-26b-ctrl and LV-miR-26b,and expressions of MALAT-1,miR-26a and miR-26b were measured by RT-PCR.The colony formation assay,CCK-8 assay,soft agar colony formation assay,scratch assay,and Transwell invasion assay were performed to detect the proliferation ability,in vitro tumorigenic capability,and migration and invasion abilities,respectively.Results The optical density values of MCF-7 cells at 24 h,48 h,72 h and 96 h in the E2 group and the Mock group were compared via the repeated measures analysis of variance,which demonstrated that they were different among the time points(P<0.05)and between the two groups(P<0.05),and that the proliferation ability of cells at 72 h and 96 h in the E2 group was higher than that in the Mock group(P<0.05).There were differences in the change trends of the optical density values between the two groups(P<0.05).Compared with the Mock group,the expression of MALAT-1 in the E2 group was higher(P<0.05),and the expressions of miR-26a and miR-26b were lower(P<0.05).Compared with the miR-26b low-expression group,the position of the survival curve of patients with breast cancer was higher in the miR-26b high-expression group,indicating a lower risk of death(P<0.05).Compared with the miR-26a low-expression group,the position of the survival curve of patients with breast cancer was lower in the miR-26a high-expression group(P<0.05).However,the risk of death was not significantly different between the two groups when including miR-26a as an independent prognostic factor in the Cox regression model(P>0.05).The expression of MALAT-1 in breast cancer cells of the LV-miR-26b-ctrl/E2 was higher than that in the LV-miR-26b-ctrl group(P<0.05),while the expression of MALAT-1 in breast cancer cells of the LV-miR-26b/E2 group was lower than that in the LV-miR

关 键 词：乳腺癌 microRNA-26b MALAT-1 雌二醇 lncRNA 浸润 转移 

分 类 号：R737.9[医药卫生—肿瘤]