宫颈腺癌患者细胞质-细胞核AR表达比率的预后意义  

Prognostic significance of the ratio of cytoplasmic- cytonuclear AR expression in patients with cervical adenocarcinoma

作　　者：郭祺 王露露 颜童 陈睿琳 周怀君[1] GUO Qi;WANG Lulu;YAN Tong;CHEN Ruilin;ZHOU Huaijun(Department of Gynecology and Obstetrics,Affiliated Drum Tower Hospital,Medical School,Nanjing University,Nanjing 210008,China)

机构地区：[1]南京大学医学院附属鼓楼医院妇产科,江苏南京210008

出　　处：《东南大学学报（医学版）》2024年第3期329-336,共8页Journal of Southeast University(Medical Science Edition)

基　　金：国家自然科学基金资助项目(81972434,82172819)。

摘　　要：目的:利用免疫组化探究雄激素受体(AR)在宫颈腺癌中的表达模式及其与预后的关系。方法:对30例因子宫腺肌症等良性疾病而接受全子宫切除术患者的正常宫颈组织标本,和86例因宫颈腺癌而接受全子宫切除术患者的肿瘤组织标本进行AR免疫组化染色,收集研究对象的一般资料,根据AR表达情况评估各临床病理特征与患者临床结局之间的关系。结果:83%(25/30)的正常宫颈组织显示AR在细胞核阳性表达,细胞质不表达,而与正常宫颈组织相比,AR在宫颈腺癌组织则呈现出不同的表达模式,94%(81/86)的患者有AR染色,其中82%(66/81)显示出核-质双染色,12%(10/81)显示出明显的单一胞质阳性,只有6%(5/81)的患者显示出与正常宫颈组织相似的单一核染色。且在核-质双染色的患者中,细胞质表达更为明显。为了更为准确地描述AR在肿瘤细胞不同亚细胞定位的表达情况,我们采用细胞质/细胞核比率对其表达进行量化。比率>2.5组更易发生淋巴结转移(P<0.01)和肌层浸润(P<0.01),且波形蛋白(vimentin)阳性率较高(P<0.01)。Kaplan-Meier分析显示,细胞质与细胞核AR表达比率越高,生存率越低。单变量和多变量Cox回归分析表明,AR胞质/胞核表达比率是远处转移的独立预后因素。结论:AR的细胞质与细胞核表达比率是一个独立的预后因素,有望成为宫颈腺癌患者远处转移风险的预测指标。Objective:To investigate the expression pattern of androgen receptor(AR) in cervical adenocarcinoma and the relationship between AR expression and prognosis using immunohistochemistry.Methods:Immunohistochemical staining for AR was performed on normal cervical tissue specimens from 30 patients who underwent total hysterectomy for benign diseases such as adenomyosis and 86 tumor tissue specimens from patients who underwent total hysterectomy for cervical adenocarcinoma.General information of the subjects was collected.The relationship between each clinicopathological feature and patient survival was assessed on the basis of AR expression.Results:83%(25/30) of normal cervical tissues showed positive nuclear staining,whereas compared to normal cervical tissues,cervical adenocarcinoma tumor tissues showed different modes of expression of AR,with 94%(81/86) of patients AR positive,of which 82%(66/81) showed nuclear-plasmic double staining,12%(10/81) showed a clear single cytoplasmic positivity,and only 6%(5/81) showed AR nuclear staining similar to normal cervical tissue.In patients with nuclear-plasmic double staining,cytoplasmic staining was more noticeable in AR.We measured the expression of AR utilizing the cytoplasmic-cytonuclear ratio in order to more precisely characterize its expression in various subcellular localizations of tumor cells.The ratio>2.5 group had more patients with positive lymph nodes(P<0.01) and deep myometrial invasion(P<0.01) compared to the ratio≤2.5 group.Additionally,the ratio>2.5 group had a higher incidence of vimentin positivity(P<0.01).Kaplan-Meier analyses revealed a higher cytoplasmic-to-cytonuclear ratio of AR expression associated with lower survival rates.Univariate and multivariate Cox regression analyses identified it as an independent prognostic factor for distant metastasis.Conclusion:The cytoplasmic-to-cytonuclear ratio of AR expression is an independent prognostic factor,offering potential as a predictor for the risk of distant metastasis in cervical adenocarcinoma patien

关 键 词：雄激素受体 质核比率 宫颈腺癌 免疫组织化学 预后 

分 类 号：R737.33[医药卫生—肿瘤]