LncRNA-TNFRSF13C调控miR-1246对LPS诱导的牙髓细胞HIF-1α及生物活性的影响  

LncRNA-TNFRSF13C regulates the effects of miR-1246 on HIF-1α and bioactivity of LPS-induced dental pulp cells

作　　者：张雪 任燕 白静 李月辉 ZHANG Xue;REN Yan;BAI Jing;LI Yue-hui(Tangshan Central Hospital,Hebei Tangshan 063000,China;Tangshan Fengrun District People's Hospital,Hebei Tangshan 063000,China;Kailuan(Group)Co.,LTD.,Tangjiazhuang Hospital,Hebei Tangshan 063100,China)

机构地区：[1]唐山中心医院,河北唐山063000 [2]唐山市丰润区人民医院,河北唐山063000 [3]开滦(集团)有限责任公司唐家庄医院,河北唐山063100

出　　处：《临床口腔医学杂志》2024年第5期263-268,共6页Journal of Clinical Stomatology

基　　金：河北省医学科学基金资助项目(编号:20230240)。

摘　　要：目的:探讨LncRNA-TNFRSF13C调控miR-1246对脂多糖(lipopolysaccharides, LPS)诱导的牙髓细胞低氧诱导因子-1α(hypoxia-inducible factor 1α,HIF-1α)及生物活性的影响机制。方法:将人牙髓细胞(human dental pulp cells, HDPCs)经LPS处理后,分为HDPCs组、TNFRSF13C NC-siRNA组、TNFRSF13C-siRNA组、sno-TNFRSF13C组、miR-NC组、miR-1246 mimics组、miR-1246 siRNA组、TNFRSF13C-siRNA+miR-1246 siRNA组。qRT-PCR检测各组细胞LncRNA-TNFRSF13C、miR-1246相对表达;免疫印迹检测各组细胞HIF-1α蛋白表达;CCK-8检测各组细胞活性;流式细胞仪检测细胞凋亡率;茜素红染色观察细胞矿化情况。结果:与HDPCs组、TNFRSF13C NC-siRNA组相比,TNFRSF13C-siRNA组HIF-1α表达量、细胞凋亡率、LncRNA-TNFRSF13C表达降低,细胞增殖率升高(P<0.05)。与miR-NC组相比,miR-1246 siRNA组HIF-1α表达量、细胞凋亡率、miR-1246表达水平降低,细胞增殖率升高(P<0.05)。与TNFRSF13C-siRNA组、miR-1246 siRNA组相比,TNFRSF13C-siRNA+miR-1246 siRNA组HIF-1α表达量、细胞凋亡率降低,细胞增殖率升高(P<0.05)。TNFRSF13C-siRNA组、miR-1246 siRNA组存在更多的矿化结节及不透光致密影;TNFRSF13C-siRNA+miR-1246 siRNA组存在大量矿化结节,致密深染。结论:抑制LncRNA-TNFRSF13C对LPS处理的HDPCs具有促进活性,降低凋亡的作用,促进HDPCs矿化,同时也可抑制HIF-1α,研究机制与调控miR-1246活性相关。Objective:To investigate the effect of LncRNA-TNFRSF13C regulation of miR-1246 on hypoxia-inducible factor 1α(HIF-1α)and bioactivity of lipopolysaccharides(LPS)-induced dental pulp cells.Methods:After human dental pulp cells(HDPCs)treated with LPS,they were divided into HDPCs group,TNFRSF13C NC-siRNA group,TNFRSF13C-siRNA group,sno-TNFRSF13C group,miR-NC group,miR-1246 mimics group,and miR-1246 siRNA group,TNFRSF13C-siRNA+miR-1246 siRNA group.The relative expressions of LncRNA-TNFRSF13C and miR-1246 were detected by qRT-PCR.The expression of HIF-1αwas detected by Western blotting.CCK-8 was used to detect cell activity in each group.The apoptosis rate was detected by flow cytometry.The mineralization of cells was observed by alizarin red staining.Results:Com-pared with HDPCs group and TNFRSF13C NC-siRNA group,HIF-1αexpression,apoptosis rate and LncRNA-TNFRSF13C expression in TNFRSF13C-SiRNA group were significantly decreased,and cell proliferation rate was significantly increased(P<0.05).Compared with miR-NC group,HIF-1αexpression,cell apoptosis rate and expression level of miR-1246 in miR-1246 siRNA group were significantly decreased,and cell proliferation rate was significantly increased(P<0.05).Compared with TNFRSF13C-siRNA group and miR-1246 siRNA group,HIF-1αexpression,cell apoptosis rate in TNFRSF13C-siRNA+miR-1246 siRNA group were significantly decreased,cell proliferation rate was significantly increased(P<0.05).TN-FRSF13C-siRNA group and miR-1246 siRNA group had more mineralized nodules and dense mass.The TNFRSF13C-siRNA+miR-1246 siRNA group had a large number of mineralized nodules,which were dense and deeply stained.Conclusion:Inhi-bition of LncRNA-TNFRSF13C can promote the activity of LPS-treated HDPCs,reduce the effect of apoptosis,promote the mineralization of HDPCs,and inhibit HIF-1α.The study mechanism is related to the regulation of miR-1246 activity.

关 键 词：牙髓炎 牙髓细胞 脂多糖 miR-1246 LncRNA-TNFRSF13C 

分 类 号：R781.3[医药卫生—口腔医学]