马铃薯组织间组成型和特异型表达基因的鉴定  

Identification of Constitutive and Specific Expression Genes Among Potato Tissues

作　　者：王荣艳 普金 罗有卫[3] 谢文娟[3] 王娟 祝光涛 WANG Rongyan;PU Jin;LUO Youwei;XIE Wenjuan;WANG Juan;ZHU Guangtao(Yunnan Provincial Key Laboratory of Potato Biology,Potato Science Institute,College of Life Sciences,Yunnan Normal University,Kunming 650500,China;Dehong Agricultural Technology Extension Center,Dehong 678400,China;College of Plant Protection,Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]云南师范大学生命科学学院,马铃薯科学研究院,云南省马铃薯生物学重点试验室,云南昆明650500 [2]云南农业大学植物保护学院,云南昆明650201 [3]德宏州农业技术推广中心,云南德宏678400

出　　处：《云南师范大学学报（自然科学版）》2024年第3期20-26,共7页Journal of Yunnan Normal University:Natural Sciences Edition

基　　金：国家自然科学基金资助项目(32272725).

摘　　要：马铃薯是重要的粮食作物,而块茎不仅是收获器官也是主栽四倍体品种的繁殖器官,近年来逐渐兴起的二倍体杂交育种通过实生种子进行繁殖,使得研究其花和果实发育变得尤为重要.因此,鉴定马铃薯不同组织组成型和组织特异表达的基因对马铃薯基因功能和遗传改良具有重要意义.通过分析二倍体马铃薯自交系A6-26和E4-63不同组织(花、块茎、根、果实、叶和茎)的转录组数据,筛选到12个组成型表达基因,同时筛选了一批花、果实和薯块中特异表达的基因,并利用GO分析和KEGG富集分析对这些基因进行了功能分析.Potatoes are important food crops,and tubers are not only the harvesting organs but also the reproductive organs of the main tetraploid varieties.Diploid hybrid breeding,which propagates through actual seeds,has gradually emerged in recent years.It has also become particularly important to study the development of flowers and fruits in potato.Therefore,identifying the constitutive expression genes of potatoes and the specific expression genes of flowers,fruits,and tubers are very important for studying potato gene function and genetic improvement.In this study,we analyzed the transcriptome data of different tissues(including flowers,tubers,roots,fruits,leaves,and stems)of diploid potato inbred lines A6-26 and E4-63 to identify 12 constitutive expression genes.And genes specifically expressed in flowers,fruits,and tubers were also screened,and their functional analysis were performed by GO analysis and KEGG enrichment analysis.This study will provide a basic theory for potato molecular biology research and genetic improvement.

关 键 词：马铃薯 组成表达 特异表达 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]