基于P&T-GC/MS指纹图谱技术的烟用香精质量控制评价方法  

Methods for quality control of tobacco flavor based on P&T-GC/MS fingerprinting technology

作　　者：吕祥敏 陈云璨 唐杰 许嘉东 蒋昊 程传玲 张改红 朱玲 LYU Xiangmin;CHEN Yuncan;TANG Jie;XU Jiadong;JIANG Hao;CHENG Chuanling;ZHANG Gaihong;ZHU Ling(Technology Center,China Tobacco Chongqing Industrial Co.,Ltd.,Chongqing 400060,China;College of Tobacco Science and Engineering,Zhengzhou University of Light Industry,Zhengzhou 450001,China)

机构地区：[1]重庆中烟工业有限责任公司技术中心,重庆400060 [2]郑州轻工业大学烟草科学与工程学院,河南郑州450001

出　　处：《轻工学报》2024年第3期54-61,共8页Journal of Light Industry

基　　金：河南省科技攻关项目(192102110212);重庆中烟工业有限责任公司科研项目(HX202206)。

摘　　要：利用P&T-GC/MS指纹图谱技术,结合夹角余弦相似度、峰匹配及多元T^(2)控制图,对烟用香精的质量稳定性进行综合评价,结果表明:P&T最优的前处理条件为吹扫时间14 min,吹扫温度40℃,解析温度200℃,在该条件下测得烟用香精中各挥发性成分特征色谱峰的RSD为1.36%~6.98%,说明该方法精密度良好;指纹图谱结合夹角余弦相似度、峰匹配分析及多元T^(2)控制图,可快速识别组分发生变化的烟用香精样品,及时发现样品的错混、质量浓度改变等问题,有效监控烟用香精的质量稳定性,并有助于进一步分析化学组分发生变化的根本原因。T o investigate t he quality stability of tobacco flavor was comprehensively evaluated using a P&T-GC/MS fingerprinting method was utilized,which involved the integration of the angle-cosine similarity analysis,peak matching,and multivariate T 2 statistics control charts.The findings suggested the following:The optimal pretreatment conditions for purge trapping involve a purge time of 14 minutes,a purge temperature of 40℃,and a desorption temperature of 200℃.By optimizing the P&T blowing and trapping conditions,a P&T-GC/MS fingerprint analysis method was established Under this condition,and the RSD of the characteristic peaks of the volatile components ranged from 1.36%to 6.98%,which indicated that the precision of the method was good.Fingerprint mapping combined with angle-cosine similarity and peak matching analysis could quickly identify tobacco flavor samples with changes in chemical components and effectively monitor the quality stability of tobacco flavor;combined with peak matching and Hotelling multivariate T 2 quality control charts,problems such as mismixing of samples and changes in substance content can could be detected in time and the quality stability of tobacco flavor could be effectively monitored.This could help to further analyze the causes of changes in chemical components,providing a powerful technical support for tobacco flavour quality control.

关 键 词：烟用香精 吹扫捕集 GC/MS 夹角余弦相似度 峰匹配 多元T^(2)控制图 

分 类 号：TS264.3[轻工技术与工程—发酵工程]