CP-31398对胆囊癌细胞增殖和转移的影响及机制  

Effect and mechanism of CP-31398 on proliferation and metastasis of gallbladder carcinoma cells

作　　者：孙学娣 曹圣芹[2] 李重阳 刘敬华 何强[4] SUN Xue-di;CAO Sheng-qin;LI Chong-yang;LIU Jing-hua;HE Qiang(Jinzhou Medical University,Jinzhou 121001,China;Department of Hand and Foot Surgery,Jinan Fourth People's Hospital,Jinan 250031,China;Binzhou Medical University,Yantai 264003,China;Department of General Surgery,Linyi People's Hospital,Linyi 276000,China)

机构地区：[1]锦州医科大学,辽宁锦州121001 [2]济南市第四人民医院手足外科,山东济南250031 [3]滨州医学院,山东烟台264003 [4]临沂市人民医院普外科,山东临沂276000

出　　处：《中国现代普通外科进展》2024年第5期364-368,共5页Chinese Journal of Current Advances in General Surgery

摘　　要：目的:研究CP-31398对表达突变型p53GBC-SD和表达野生型p53NOZ胆囊癌细胞株增殖和转移的影响。方法:突变型p53GBC-SD中加入药物CP-31398为CP-31398组,未加药组为对照组;野生型p53NOZ中加入药物CP-31398为CP-31398组,未加药组为对照组。利用CCK8细胞增殖实验检测CP-31398对野生型及突变型p53胆囊癌细胞增殖的影响;利用流式细胞术检测CP-31398对胆囊癌细胞周期及凋亡的影响;利用Transwell实验检测CP-31398对胆囊癌细胞迁移和侵袭的影响;利用Western blot检测CP-31398对p53蛋白表达情况的影响,并对相关机制进行探讨。结果:在突变型p53GBC-SD细胞中,CCK8细胞增殖实验显示CP-31398组较对照组胆囊癌细胞的增殖速度减慢(P<0.01);周期实验显示与对照组相比,CP-31398组的细胞处在S期的细胞比例上升,处在G0、G1期的细胞比例下降,所以细胞在S期被阻断(P<0.001);凋亡实验显示对照组的凋亡率明显低于CP-31398组(P<0.05);Transwell实验显示,对照组穿过小室的细胞数目高于CP-31398组;Western blot实验说明CP-31398组p53蛋白表达量增加(P<0.05)。在表达野生型p53NOZ细胞中,CCK8细胞增殖实验显示CP-31398组较对照组胆囊癌细胞的增殖速度减慢(P<0.001);周期实验显示处在G2、M期的细胞比例明显上升,处于G0、G1期的细胞比例下降,细胞被阻滞于G2、M期(P<0.001);凋亡实验显示对照组的凋亡率是(14.04±3.08)%,CP-31398组的凋亡率是(34.55±3.30)%(P<0.01);Transwell实验显示,对照组穿过小室的细胞数目高于CP-31398组;Western blot实验显示CP-31398组p53蛋白表达量增加(P<0.01)。结论:CP-31398有效抑制胆囊癌细胞的增殖及转移,并且不依赖p53的突变,且CP-31398对表达野生型p53的NOZ胆囊癌细胞株作用效果较好,可以作为胆囊癌药物治疗的一个新选择。Objective:To investigate the effects of CP-31398 on proliferation and metastasis of gallbladder carcinoma cell lines expressing mutant P53GBC-SD and wild-type p53NOZ.Methods:The effects of CP-31398 on the proliferation of wild-type and mutant p53 gallbladder carcinoma cells were detected by CCK8 cell proliferation assay and clonal formation assay.The effect of CP-31398 on cell cycle and apoptosis of gallbladder carcinoma was detected by flow cytometry.The effects of CP-31398 on the migration and invasion of gallbladder carcinoma cells were detected by scratch,cytoskeleton and Transwell assay.The effect of CP-31398 on the expression of p53 protein was detected by Western blot and the related mechanism was discussed.Results:In mutant P53GBC-SD cells,the proliferation of CCK8 cells showed that the proliferation rate of gallbladder cancer cells in CP-31398 treatment group was slower than that in control group(P<0.01).The cycle experiment showed that compared with the control group,the proportion of cells in S phase increased in CP-31398 treatment group,and the proportion of cells in G0 and G1 phase decreased,so the cells were blocked in S phase(P<0.001).Apoptosis experiment showed that the apoptosis rate of control group was lower than CP-31398 treatment group(P<0.05).Transwell experiment showed that the number of cells passing through the cell compartment in the control group was higher than that in the CP-31398 treatment group.Western blot analysis showed that the expression of p53 protein in CP-31398 treatment group was increased(P<0.05).In wild-type p53NOZ cells,the proliferation of CCK8 cells showed that the proliferation rate of gallbladder cancer cells in CP-31398 treatment group was slower than that in control group.The number of cell clones in the control group was higher than that in the CP-31398 treatment group(P<0.001).The cycle test showed that the proportion of cells in G2 and M phases increased significantly,while the proportion of cells in G0 and G1 phases decreased,and the cells were blocked in G2 an

关 键 词：胆囊肿瘤 P53突变 CP-31398 细胞增殖 肿瘤转移 

分 类 号：R735.8[医药卫生—肿瘤]