安罗替尼对脑胶质瘤T98G细胞迁移侵袭及上皮间质转化的抑制作用  

Inhibitory effect of anlotinib on migration,invasion and epithelial-mesenchymal transition of glioma T98G cells

作　　者：柳新 张圣林[1] 李青山[1] 董怡 谢云鹏[2] LIU Xin;ZHANG Shenglin;LI Qingshan;DONG Yi;XIE Yunpeng(Department of Oncology,Affiliated Hospital of Chengde Medical College,Chengde 067000,China;不详)

机构地区：[1]承德医学院附属医院肿瘤科,承德067000 [2]承德医学院附属医院神经外科,承德067000

出　　处：《临床神经外科杂志》2024年第3期286-291,共6页Journal of Clinical Neurosurgery

基　　金：河北省卫生健康委员会医学科学研究基金资助项目(20231363)。

摘　　要：目的探究安罗替尼对人脑胶质瘤细胞黏附、迁移、侵袭和上皮间质转化(EMT)的影响。方法体外培养人脑胶质瘤T98G细胞,分为对照组(不做干预)、实验组(2.5μmol·L^(-1)安罗替尼组、5μmol·L^(-1)安罗替尼组、10μmol·L^(-1)安罗替尼组)和阳性药物组(50 mg·L^(-1)5-氟尿嘧啶),干预24 h。用活细胞计数(CCK-8)、细胞黏附实验、划痕法、Transwell小室及蛋白免疫印迹(WB)法对细胞增殖活力、黏附、迁移、侵袭能力及EMT相关蛋白表达量进行分析。结果与对照组比较,5μmol·L^(-1)、10μmol·L^(-1)安罗替尼组和阳性药物组增殖活力显著降低(P<0.05),实验组和阳性药物组T98G细胞黏附、迁移、侵袭能力,纤维粘连蛋白(FN)、波形蛋白(Vimentin)、N-钙黏蛋白(N-cadherin)蛋白的表达水平呈现显著下调趋势(P<0.05),而E-钙黏蛋白(E-cadherin)蛋白的表达水平呈现明显上调趋势(P<0.05),且浓度越高变化越显著;与阳性药物组相比,2.5μmol·L^(-1)、5μmol·L^(-1)安罗替尼组细胞增殖活力、黏附、迁移、侵袭能力,N-cadherin、Vimentin及FN的蛋白水平上调(P<0.05),而E-cadherin蛋白水平则下调(P<0.05);10μmol·L^(-1)安罗替尼组与阳性药物组相比无显著差异(P>0.05)。结论安罗替尼可能是通过抑制EMT进程进而抑制人脑胶质瘤T98G细胞的增殖、黏附、迁移、侵袭能力。Objective To investigate the effects of anlotinib on the adhesion,migration,invasion and epithelial-mesenchymal transition(EMT)of human glioma cells.Methods Human glioma T98G cells were cultured in vitro and divided into control group(no intervention),experimental group(2.5μmol·L^(-1)anlotinib group,5μmol·L^(-1)anlotinib group,10μmol·L^(-1)anlotinib group)and positive drug group(50 mg·L^(-1)5-fluorouracil)for 24 hours.Cell proliferation,adhesion,migration,invasion and EMT-related protein expression were analyzed by cell count(CCK-8),cell adhesion assay,scratch assay,Transwell and Western blotting(WB).Results Compared with the control group,the proliferative activity of 5μmol·L^(-1),10μmol·L^(-1)and positive drug groups was significantly decreased(P<0.05),and the adhesion,migration and invasion ability of T98G cells in the experimental group and positive drug group were significantly decreased(P<0.05).The expression levels of fibrin(FN),Vimentin and N-cadherin were significantly down-regulated(P<0.05),while the expression levels of E-cadherin were significantly up-regulated(P<0.05).The higher the concentration,the more significant the change.Compared with positive drug group,the cell proliferation activity,adhesion,migration invasion ability,and the protein levels of N-cadherin,Vimentin and FN were up-regulated,while the protein level of E-cadherin was down-regulated in the 2.5μmol·L^(-1)and 5μmol·L^(-1)anlotinib groupsd(P<0.05).There was no significant difference between the 10μmol·L^(-1)antirotinib group and the positive group(P>0.05).Conclusion Anlotinib may inhibit the proliferation,adhesion,migration and invasion ability of human glioma T98G cells by inhibiting the process of EMT.

关 键 词：安罗替尼 脑胶质瘤 黏附 迁移 侵袭 上皮间质转化 

分 类 号：R739.41[医药卫生—肿瘤]