补肾化痰方含药血清对HepG2肝癌细胞增殖、迁移的实验研究  

Experimental study on the proliferation and migration of drug containing serum of bushenhuatan formulae on HepG2 hepatoma cells

作　　者：宋幸铃 周亚娜[2,3,4] 许海 罗保平 周用[2,3,4] 左盼 田晓霞[1] 魏如薇 SONG Xing-ling;ZHOU Ya-na;XU Hai(Hubei University of Chinese Medicine(Wuhan Hubei,430065),China;不详)

机构地区：[1]湖北中医药大学第一临床学院,湖北武汉430065 [2]湖北省中医院 [3]湖北中医药大学附属医院 [4]湖北省中医药研究院 [5]湖北中医药大学黄家湖医院妇科

出　　处：《中西医结合肝病杂志》2024年第6期526-529,540,共5页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：湖北省自然科学基金(No.2022CFB542);湖北中医药大学十四五优秀学科团队项目(中医校[2022]90号);省级区域中医医疗综合中心建设专项(No.P-20230901-1229);湖北中医药大学大学生创新创业训练计划省级项目(No.5432-10050308)。

摘　　要：目的:研究补肾化痰方(BHF)对小鼠脏器指数及BHF含药血清对HepG2肝癌细胞增殖、迁移的影响。方法:将小鼠随机分为对照组、BHF高剂量组、BHF中剂量组、BHF低剂量组,灌胃7 d后,采血制备含药血清,处死后取材测定各组小鼠脏器指数。体外培养HepG2肝癌细胞,设置空白组、对照组、BHF高剂量组、BHF中剂量组、BHF低剂量组,每组设3个浓度(20%、10%、5%),3个复孔。各组加入相应的含药血清,培养24、48、72 h后,采用CCK8法测算细胞增殖抑制率。采用Transwell细胞培养24 h后镜下观察各组细胞的形态学改变。进行细胞划痕实验,检测各组HepG2细胞迁移的水平。结果:在20%血清浓度下,与对照组比较,24 h时BHF高、低剂量组细胞增殖抑制率显著增加(P<0.05)。72 h时BHF各剂量组细胞增殖抑制率均显著增加(P<0.05),且高、低剂量组均高于中剂量组(P<0.05)。在10%血清浓度下,与对照组比较,24 h时BHF低剂量组细胞增殖抑制率显著增加(P<0.05),72 h时BHF各剂量组细胞增殖抑制率均显著增加(P<0.05),且高剂量组显著高于低剂量组(P<0.05)。在5%血清浓度下,与对照组比较,48 h时BHF高剂量组显著增加(P<0.05)。72 h时BHF各剂量组细胞增殖抑制率均显著增加(P<0.05),且高剂量组显著高于中、低剂量组(P<0.05)。与空白组比较,72 h时BHF高剂量组划痕迁移率显著降低(P<0.05)。结论:BHF含药血清对HepG2肝癌细胞增殖、迁移具有抑制作用,且高剂量组表现最优。Objective:To study the effects of Bushenhuatan Formulae(BHF)on organ index of mice and BHF-containing serum on the proliferation and migration of HepG2 hepatocellular carcinoma cells.Methods:Mice were randomly divided into control group,BHF high-dose group,BHF medium-dose group,BHF low-dose group,and after gavage for 7 days,blood was collected to prepare drug-containing serum,and samples were taken to determine the organ index of mice in each group after execution.HepG2 hepatocellular carcinoma cells were cultured in vitro,and the blank group,control group containing drug-containing serum,BHF high-dose group containing drug-containing serum,BHF medium-dose group containing drug-containing serum,BHF low-dose group containing drug-containing serum were set up with three concentrations in each group(20%,10%,and 5%),and 3 replicate wells were set up in each group.The level of cell proliferation in each group was detected by CCK8 method after 24 h,48 h and 72 h of culture after adding the corresponding drugs in each group.Morphological changes of cells in each group were observed microscopically after 24 h of culture using transwell cells.The subsequent experiments were carried out after screening the optimal effector-containing serum concentration.The level of HepG2 cell migration in each group was detected using cell scratch assay.Results:Cell proliferation showed that at 24 h,the cell proliferation inhibition rate was significantly lower in the BHF low-dose group than in the control group(P<0.05),the BHF high-dose group(P<0.01),and the BHF medium-dose group(P<0.01).At 48 h,the cell proliferation inhibition rate in the BHF medium-dose group was significantly higher when compared with that in the control group(P<0.05),and significantly higher than that in the BHF low-dose group(P<0.01).At 72 h,the cell proliferation inhibition rate was significantly higher in all BHF dose groups compared with the control group(P<0.01).And the cell inhibition rate of BHF high dose group was significantly higher than that of BHF middle

关 键 词：补肾化痰方 肝癌 含药血清 细胞增殖 细胞迁移 

分 类 号：R735.7[医药卫生—肿瘤]