GRWD1对胃癌细胞增殖和侵袭能力的影响  

Effect of GRWD1 on the proliferation and invasion of gastric cancer cells

作　　者：黄建平[1] 吴雪晶[1] 郑宇辉[1] 李小燕[1] 施栋梁 杨映红[1] HUANG Jianping;WU Xuejing;ZHENG Yuhui;LI Xiaoyan;SHI Dongliang;YANG Yinghong(Department of Pathology,the Affiliated Union Hospital,Fujian Medical University,Fruzhou,Fujian 350001,China)

机构地区：[1]福建医科大学附属协和医院病理科,福州350001

出　　处：《福建医药杂志》2024年第3期5-8,共4页Fujian Medical Journal

基　　金：福建省自然科学基金(2022J01719)。

摘　　要：目的探讨富含谷氨酸的WD重复序列蛋白1(glutamate-rich WD40 repeat containing1,GRWD1)蛋白表达水平对胃癌MKN-45细胞增殖和侵袭能力的影响.方法分别构建GRWD1过表达载体和设计siRNA并转染胃癌MKN-45细胞,设立GRWD1过表达组及对照组、siRNA基因沉默组及对照组.采用CCK-8法测定各组细胞增殖差异,采用transwell检测GRWD1过表达和基因沉默对胃癌细胞MKN-45侵袭能力的影响.结果siRNA组转染MKN-45细胞24h后,细胞增殖水平和侵袭能力明显受到抑制(P<0.01),过表达组转染MKN-45细胞24h后,细胞增殖水平和侵袭能力明显增强(P<0.01).结论GRWD1与MKN-45胃癌细胞增殖和侵袭能力有关,下调GRWD1可抑制MKN-45细胞增殖水平和侵袭能力.反之,上调GRWD1可提高MKN-45细胞增殖水平和侵袭能力.Objective To investigate the effect of GRWD1(Glutamate rich WD40 repeat containing 1)on the proliferation and invasion ability of gastric cancer MKN-45 cells.Methods GR WD1 overexpression vector and siRNA were constructed and transfected into gastric cancer MKN-45 cells.GRWD1 overexpression group,siRNA group and control group were established.Cell proliferation levels in each group were measured using the CCK-8,and transwell was used to detect the effects of GRWD1 overexpression and gene silencing on the invasion ability of gastric cancer cell line MKN-45.Results The results showed that after transfection of MKN-45 cells with siRNA for 24 hours,the cell proliferation level and invasion ability were significantly inhibited(P<0.01),while after transfection of MKN 45 cells with overexpression vector for 24 hours,the cell proliferation level and invasion ability were significantly enhanced(P<0.01).Conclusion GRWD1 is related to the proliferation and invasion ability of MKN-45 gastric cancer cells,and downregulation of GR WD1 can inhibit the proliferation level and invasion ability of MKN-45 cells.On the contrary,upregulation of GRWD1 can improve the proliferation level and invasion ability of MKN-45 cells.

关 键 词：GRWD1 胃癌 增殖 侵袭 

分 类 号：R735.2[医药卫生—肿瘤]