NCAPG对大肠癌细胞HCT116增殖和凋亡的影响  

Effects of NCAPG on the proliferation and apoptosis of colorectal cancer cell HCT116

作　　者：汪家航 林明强 张捷平[2] WANG Jiahang;LIN Mingqiang;ZHANG Jieping(Department of Gastrointestinal Surgery,Fuzhou Second General Hospital,Fuzhou,Fujian 350025,China;Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian 350000,China)

机构地区：[1]福州市第二总医院胃肠外科,福州350025 [2]福建中医药大学,福州350122

出　　处：《福建医药杂志》2024年第3期94-99,共6页Fujian Medical Journal

基　　金：2021年福州市卫生健康中青年科学研究项目(2021-S-wq19);福建省创伤骨科急救与康复临床医学研究中心项目(2020Y2014)。

摘　　要：目的探讨非染色体结构维持蛋白复合体IG亚基(Non-SMC condensin I complex subunitG,NCAPG)对大肠癌细胞生长的作用和对增殖凋亡的影响.方法敲减NCAPG的小干扰RNA(si-NCAPG)转染人大肠癌细胞株HCT116,Western blot检测si-NCAPG对大肠癌细胞中NCAPG蛋白表达的影响;通过观察细胞形态、细胞活力评价si-NCAPG对大肠癌细胞生长的影响,并筛选效果最好的序列用于后续实验.通过克隆形成、细胞周期和凋亡、Western blot实验评价si-NCAPG对大肠癌细胞生长、周期和凋亡的影响.结果si-NCAPG显著降低大肠癌细胞中NCAPG的蛋白表达(P<0.05),抑制大肠癌细胞数目和细胞活力(P<0.05),使细胞阻滞在G2/M期(P<0.05),并促进细胞凋亡(P<0.05).敲减NCAPG降低CyclinB1、CDK1和Bcl-2蛋白表达水平和升高BAX的蛋白表达水平(P<0.05).结论敲减NCAPG通过阻滞细胞在G2/M期和促进细胞凋亡,达到抑制大肠癌细胞生长的作用.Objective To investigate the effect and mechanism of Non SMC condensin I complex subunit G(NCAPG)on the growth,proliferation and apoptosis of colorectal cancer cells(CRC).Methods siRNA-NCAPG was transfected into human colorectal cancer cell line HCT116 cells.The effect of si-NCAPG on the expression of NCAPG in HCT116 cells was detected by Western blot.The effect of si NCAPG on the growth of HCT116 cells was evaluated by observing cell morphology and:ell viability,and the best sequences were screened for subsequent experiments.The effects of si-NCAPG on the cell growth,cycle and apoptosis of HCT116 cells were evaluated by clonal formation,cell cycle,apoptosis,and western blot.Results siNCAPG significantly decreased the expression of NCAPG protein level(P<0.05),inhibited the cell number and cell viability(P<0.05),induced cell cycle arrest in G2/M phase(P<0.05)and promoted cell apoptosis in HCT116 cells(P<0.05).K nocking down NCAPG reduced expression levels of Cyclin B1,CDK1 and Bcl-2 protein and increased expression levels of BAX protein.Conclusion si NCAPG significantly inhibits the growth of CRC by inducing cell cycle arrest at G2/M phase and promoting cell apoptosis.

关 键 词：非染色体结构维持蛋白复合体IG亚基 大肠癌 细胞生长 凋亡 

分 类 号：R735.34[医药卫生—肿瘤]