自组装脂质体纳米载体对骨肉瘤细胞Saos-2靶向递送及凋亡的影响  

Study on targeted delivery and apoptosis effects of self-assembled liposome nanocarriers on osteosarcoma cells Saos-2

作　　者：陈康尧 CHEN Kangyao(Department of Orthopaedics,Fuzhou Second General Hospital,Fuzhou,Fujian 350007,China)

机构地区：[1]福建省福州市第二总医院骨科,福州350007

出　　处：《福建医药杂志》2024年第3期103-106,共4页Fujian Medical Journal

基　　金：福州市科技计划项目(2021-S-166);福建省创伤骨科急救与康复临床医学研究中心项目(2020Y2014)。

摘　　要：目的以天然脂肪酸月桂酸和硬脂酸为骨架,利用它们在二甲基甲酰胺(dimethylformamide,DMF)中的自组装构建了一种脂质体纳米载体并负载阿霉素,对其靶向人骨肉瘤细胞Saos-2及凋亡情况进行考察.方法采用自组装制备了负载阿霉素的脂质体纳米载体,采用激光共聚焦考察了其靶向人骨肉瘤细胞Saos-2能力,采用Annexin V-FITC染色技术考察了脂质体纳米载体对人骨肉瘤细胞Saos-2凋亡的影响.结果自组装脂质体纳米载体与人骨肉瘤细胞Saos-2孵育48h时,荧光强度最高,绿色荧光的自组装脂质体纳米载体会围绕在骨肉瘤细胞Saos-2周围,相对于同剂量DMF的对照组,自组装脂质体纳米载体组的细胞无论是早期凋亡还是晚期凋亡的比例均有所增加.结论负载阿霉素的脂质体纳米载体能准确地靶向人骨肉瘤细胞Saos-2,并加速人骨肉瘤细胞Saos-2的凋亡.Objective Using natural fatty acids lauric acid and stearic acid as the backbone,a liposome nanocarrier was constructed through their self assembly in dimethylformamide(DMF)and loaded with doxorubicin.Its targeting ability to human osteosarcoma cells Saos 2 and apoptosis were investigated.Methods Doxorubicin loaded liposome nanocarriers were prepared by self assembly.The targeting ability of the nanocarriers to human osteosarcoma cells Saos 2 was examined using confocal laser scanning microscopy.The effect of liposome nanocarriers on the apoptosis of human osteosarcoma cells Saos-2 was investigated using Annexin V-FITC staining technique.Results When the self assembled liposome nanocarriers were incubated with human osteosarcoma cells Saos-2 for 48 hours,the fluorescence intensity was the highest.The green fluorescent self assembled liposome nanocarriers surrounded the osteosarcoma cells Saos2.Compared to the control group with the same dose of DMF,both early and late apoptotic cell proportions increased in the self assembled liposome nanocarrier group.Conclusion Doxorubicin-loaded liposome nanocarriers can accurately target human osteosarcoma cells Saos-2 and accelerate their apoptosis.

关 键 词：脂质体纳米载体 阿霉素 骨肉瘤细胞Saos-2 细胞靶向 细胞凋亡 

分 类 号：R738.1[医药卫生—肿瘤]