血清中LncRNA NEAT1和SP1与帕金森病患者H-Y分级的相关性及诊断意义  

Correlation and diagnostic significance of serum LncRNA NEAT_(1) and SP1 with H-Y grade in patients with Parkinson's disease

作　　者：刘越存 刘清霞 刘琦 LIU Yuecun;LIU Qingxia;LIU Qi(Department of Neurology,Binhaiwan Central Hospital of Dongguan,Dongguan,Guangdong 523900,China)

机构地区：[1]东莞市滨海湾中心医院神经内科,广东东莞523900

出　　处：《中华全科医学》2024年第6期983-986,共4页Chinese Journal of General Practice

基　　金：广东省医学科研基金项目(A2022335);东莞市社会发展科技项目(20211800900492)。

摘　　要：目的探讨帕金森病(PD)患者血清中长链非编码RNA核旁斑组装转录本1(LncRNA NEAT1)和特异性蛋白1(SP1)的表达水平与疾病严重程度的关系。方法选取2020年11月—2022年1月东莞市滨海湾中心医院收治的80例PD患者(PD组),根据修订版Hoehn-Yahr(H-Y)分级分为轻度PD亚组26例、中度PD亚组31例、重度PD亚组23例,选取同期57名体检健康者作为对照组。比较各组血清LncRNA NEAT1、SP1 mRNA表达差异及与H-Y分级的相关性,采用ROC曲线分析两者表达对PD的诊断价值。结果PD组血清LncRNA NEAT1、SP1 mRNA表达高于对照组(t=6.887、6.303,P<0.001)。轻度、中度、重度PD亚组血清LncRNA NEAT1、SP1 mRNA表达依次升高(F=66.803、H=59.259,P<0.001)。PD患者血清LncRNA NEAT1、SP1 mRNA表达与H-Y分级呈正相关关系(rs=0.790、0.840,P<0.001),两者单独与联合诊断PD的曲线下面积分别为0.787、0.784、0.858,灵敏度分别为58.75%、51.25%、70.00%,特异度分别为91.23%、96.49%、89.47%。结论PD患者血清LncRNA NEAT1、SP1 mRNA表达升高与病情进展密切相关,LncRNA NEAT1、SP1的表达水平对PD具有一定的诊断价值,且联合诊断价值更高。Objective To investigate the expression levels of long non-coding RNA para-plaque assembly transcript 1(LncRNA NEAT_(1))and SP 1 in the serum of patients with Parkinson's disease(PD)and their relationship with disease severity.Methods A total of 80 patients with PD(PD group)admitted to Binhaiwan Central Hospital of Dongguan from November 2020 to January 2022 were selected and divided into mild PD subgroup(n=26),moderate PD subgroup(n=31)and severe PD subgroup(n=23)according to the revised Hoehn-Yahr(H-Y)classification,and 57 healthy subjects(control group)were selected during the same period.The differences in serum LncRNA NEAT_(1),and SP 1 mRNA expression in each group and their correlation with the H-Y stage were compared.The ROC curve was used to analyze the diagnostic value of the expression of lncRNA NEAT_(1) and SP 1 in PD.Results The serum levels of LncRNA NEAT_(1) and SP 1 mRNA in the PD group were higher than those in the control group(t=6.887,6.303,all P<0.001).The serum levels of LncRNA NEAT_(1) and SP 1 mRNA in mild,moderate,and severe PD subgroups increased in turn(F=66.803,H=59.259,all P<0.001).The expressions of serum LncRNA NEAT_(1) and SP 1 mRNA in PD patients were positively correlated with the H-Y stage(r s=0.790,0.840,P<0.001),and the areas under the curve were 0.787,0.784,and 0.858,respectively.The sensitivity was 58.75%,51.25%and 70.00%,and the specificity was 91.23%,96.49%and 89.47%,respectively.Conclusion The increased expression of serum LncRNA NEAT_(1) and SP 1 mRNA in PD patients is closely related to the progression of the disease,which has a certain diagnostic value for PD,and the combined diagnostic value is higher.

关 键 词：帕金森病 长链非编码RNA核旁斑组装转录本1 特异性蛋白1 诊断 

分 类 号：R743[医药卫生—神经病学与精神病学] R446.7[医药卫生—临床医学]