甲基化转移酶SETDB1对口腔鳞状癌细胞迁移侵袭能力的影响及其机制  

Effect of methyltransferase SETDB1 on migration and invasion of oral cancer cells and its mechanism

作　　者：郝妍[1] 白相宇 霍峰[1] 陈喜波[1] HAO Yan;BAI Xiangyu;HUO Feng;CHEN Xibo(Department of Stomatology,Affiliated Hospital of Chengde Medical University,Chengde 067000,China)

机构地区：[1]承德医学院附属医院口腔科,河北承德067000

出　　处：《山东医药》2024年第17期21-24,共4页Shandong Medical Journal

基　　金：河北省医学科学研究课题计划项目(20210143)。

摘　　要：目的观察甲基化转移酶SETDB1对口腔鳞状癌细胞迁移、侵袭能力的影响,并探讨其相关机制。方法以不同浓度(0、0.2、0.4、0.6、0.8μmol/L)甲基化转移酶抑制剂GSK3685032作用于口腔鳞状癌细胞CAL-27,MTT法测算细胞增殖活力,筛选得到GSK3685032最佳作用浓度为0.6μmol/L。将CAL-27细胞分为对照组与实验组,对照组加入有机溶剂DMSO进行处理,实验组加入0.6μmol/L的GSK3685032处理。采用RT-qPCR法检测细胞SETDB1 mRNA,Western blotting法检测细胞SETDB1蛋白,细胞划痕实验观察细胞迁移能力,Transwell小室侵袭实验观察细胞侵袭能力,焦磷酸测序检测细胞内SOX7甲基化水平。结果实验组细胞SETDB1 mRNA及蛋白表达均低于对照组(P均<0.05);实验组细胞迁移距离小于对照组,穿膜细胞数少于对照组(P均<0.05);实验组细胞内SOX7甲基化率小于对照组(P<0.05)。结论甲基化转移酶SETDB1可抑制口腔鳞状癌细胞的迁移、侵袭能力,其机制可能与下调细胞内SOX7甲基化水平有关。Objective To observe the effect of methyltransferase SETDB1 on the migration and invasion of oral squamous cell carcinoma cells,and to explore the related mechanisms.Methods Different concentrations of methyltransferase inhibitors GSK3685032(0,0.2,0.4,0.6,0.8μmol/L)acted on the oral squamous cell carcinoma CAL-27 cells.Cell proliferative viability was measured by the MTT method,and we found that the optimal action concentration of GSK3685032 was 0.6μmol/L.CAL-27 cells were divided into the control and experimental groups;cells in the control group were treated with organic solvent DMSO,and cells in the experimental group were treated with 0.6μmol/L GSK3685032.Cell SETDB1 mRNA was measured by RT-qPCR,and the SETDB1 protein was detected by Western blotting.Cell migration ability was detected by Scratch assay,the invasion ability was detected by Transwell chamber invasion assay,and SOX 7 methylation level was tested by pyrosequencing.Results The mRNA and protein expression levels of SETDB1 were lower in the experimental group than in the control group(both P<0.05).The migration distance of the experimental group was less than that of the control group,and the number of transmenbrance cells was smaller than that of the control group(P<0.05).The methylation rate of SOX 7 in the experimental group was lower than that of the control group(P<0.05).Conclusion Methyltransferase SETDB1 can inhibit the migration and invasion of oral squamous cell carcinoma cells,and the mechanism may be related to the down-regulation of intracellular SOX7 methylation level.

关 键 词：甲基化转移酶 SETDB1 甲基化转移酶抑制剂 SOX7 口腔癌 口腔鳞状细胞癌 

分 类 号：R739.8[医药卫生—肿瘤]