过/降表达circSTON2的胰腺癌细胞增殖、迁移、侵袭能力观察  

Proliferation,migration and invasion abilities of pancreatic cancer cells with over-/down-expression of circSTON2

作　　者：叶伟标[1] 郑银海 李广柳 李伟滔 徐咏强 YE Weibiao;ZHENG Yinhai;LI Guangliu;LI Weitao;XU Yongqiang(Dongguan Clinical and Pathological Diagnosis Center,The Tenth Affiliated Hospital,Southern Medical University,Dongguan 523059,China;不详)

机构地区：[1]南方医科大学附属第十医院东莞临床病理诊断中心,广东东莞523059 [2]广东医科大学附属医院病理科

出　　处：《山东医药》2024年第17期25-28,共4页Shandong Medical Journal

基　　金：广东省医学科研基金项目(C2018054)。

摘　　要：目的观察过/降表达环状RNA-STON2(circSTON2)的胰腺癌细胞株增殖、迁移及侵袭能力变化,初步探讨circSTON2在胰腺癌中的生物学功能。方法体外传代培养人胰腺癌细胞株PANC-1,分为circSTON2过表达组、circSTON2降表达组及对照组,利用质粒转染及siRNA干扰技术分别转染circSTON2过表达质粒、circSTON2-siRNA干扰质粒及空白对照质粒。采用CCK-8法检测培养24、36、48 h时细胞增殖情况,Transwell实验检测细胞迁移和侵袭能力。结果培养24、36、48 h时,细胞增殖能力circSTON2过表达组>对照组>circSTON2降表达组(P均<0.05);迁移细胞数、侵袭细胞数circSTON2过表达组>对照组>circSTON2降表达组(P均<0.05)。结论circSTON2过表达可增强胰腺癌细胞的增殖、迁移和侵袭能力,而circSTON2降表达后作用与之相反。Objective To observe change of proliferation,migration and invasion abilities of pancreatic cancer cells with over-/down-expression of circSTON2,and to preliminarily investigate the biological function of circSTON2 in pancreatic cancer.Methods Human pancreatic cancer cell line PANC-1 was in vitro cultured.CircSTON2 over-expression group,circSTON2 down-expression group,and control group were established.Plasmid transfection and siRNA interference were used to transfect the circSTON2 overexpression plasmid and circSTON2-siRNA interference plasmid and blank control plasmid into the above groups.At 24,36 and 48 hours of culture,CCK-8 assay was used to detect cell proliferation and Transwell assay was used to detect cell migration and invasion.Results At 24,36 and 48 hours of culture,cell proliferation ability was as follows:circSTON2 over-expression group>control group>circSTON2 down-expression group(all P<0.05);the number of migration cells and the number of invasion cells were as follows:circSTON2 over-expression group>control group>circSTON2 down-expression group(all P<0.05).Conclusions The over-expression of circSTON2 promotes proliferation,migration,and invasion of pancreatic cancer cells.However,the effect of down-expression of circSTON2 is the opposite.

关 键 词：环状RNA-STON2 胰腺癌 PANC-1细胞 细胞迁移 细胞侵袭 

分 类 号：R735.9[医药卫生—肿瘤]