重瓣榆叶梅全叶绿体基因组遗传特征分析  

Genetic characteristics of whole chloroplast genome in Prunus triloba‘Multiplex’

作　　者：段春燕[1] 王晓凌[1] DUAN Chunyan;WANG Xiaoling(College of Agriculture/Peony College,Henan University of Science and Technology,Luoyang 471000,Henan,China)

机构地区：[1]河南科技大学农学院/牡丹学院,河南洛阳471000

出　　处：《浙江农林大学学报》2024年第3期577-585,共9页Journal of Zhejiang A&F University

基　　金：河南省特色骨干学科建设项目(17100001);河南科技大学博士科研启动基金资助项目(4024-13480114)。

摘　　要：【目的】对重瓣榆叶梅Prunus triloba‘Multiplex’全叶绿体基因组序列进行测序,探究其系统发育位置并分析其叶绿体基因组成特点。【方法】以重瓣榆叶梅叶片为材料,采用2×CTAB法提取叶绿体DNA,利用Illumina NovaSeq平台进行叶绿体基因组的测序,组装、注释并分析其叶绿体基因组遗传特征。联合美国国家生物技术信息中心(NCBI)数据库数据,基于全叶绿体基因组序列构建了重瓣榆叶梅系统进化关系。【结果】重瓣榆叶梅叶绿体基因组全长为157827 bp,NCBI登录号MT937181,其结构为经典的四分体结构,由1个大单拷贝区域(LSC),1个小单拷贝区域(SSC)及反向重复区域(IRa/IRb)构成,其序列长度分别为86032、19023、26386 bp。GC和AT的总占比分别为36.80%和63.20%。重瓣榆叶梅的完整叶绿体基因组序列注释到132个基因,包括tRNA基因、编码蛋白基因、rRNA基因,分别为37、87、8个。重瓣榆叶梅叶绿体基因组共编码26678个密码子和236个符合条件的SSR位点。SSR位点中A/T碱基占优势,碱基偏好性十分明显。【结论】系统进化树分析表明,重瓣榆叶梅和榆叶梅P.triloba聚合成一分支结构,与同属植物长柄扁桃P.pedunculata亲缘关系较近。[Objective]To investigate the phylogenetic location of Prunus triloba‘Multiplex’and analyze its chloroplast gene composition characteristics,the whole chloroplast genome sequence of P.triloba‘Multiplex’was sequenced.[Method]The leaves of P.triloba‘Multiplex’were used as materials to extract chloroplast DNA using 2×CTAB method.The whole chloroplast genome was sequenced by Illumina NovaSeq platform.We then assembled,annotated,and analyzed the genetic characteristics of the chloroplast genomic sequence.Combined with the NCBI database data,the phylogenetic relationship of P.triloba‘Multiplex’was constructed based on the whole chloroplast genome sequence.[Result]The total length of the chloroplast genome of P.triloba‘Multiplex’is 157827 bp,with NCBI registration number MT937181.The structure is a classic tetrad structure,consisting of 1 LSC(large single copy region),1 SSC(small single copy region),and IRa/IRb(reverse repeat region),with sequence lengths of 86032,19023 and 26386 bp,respectively.The total content of GC and AT is 36.80%and 63.20%,respectively.The complete chloroplast genome sequence of P.triloba‘Multiplex’was annotated to 132 genes,including 37 tRNA genes,87 coding protein genes,and 8 rRNA genes,respectively.The chloroplast genome of P.triloba‘Multiplex’encodes a total of 26 678 codons and 236 eligible SSR loci. The A/T base is dominant in the SSR site, and the base preference is very obvious. [Conclusion] The phylogenetic tree analysis showed that P. triloba ‘Multiplex’ and P. triloba aggregate into a branch structure, and are closely related to P. pedunculata in the same genus. The results of this study will provide new evidence and basic experimental data for species identification, phylogenetic evolution, and breeding and selection of P. triloba ‘Multiplex’.

关 键 词：重瓣榆叶梅 全叶绿体基因组 密码子偏好性 重复序列 系统发育 

分 类 号：Q943.2[生物学—植物学]