胶质瘤组织lncRNA SNHG25、miR-497-5p表达与临床特征及预后的关系研究  

Relationship between the expression of lncRNA SNHG25 and miR-497-5p in glioma tissues and clinical features and prognosis

作　　者：段晓伟[1] 张宁[1] 王晶[1] 高立威[1] 刘秀杰[1] 王喜旺[1] 于国渊[1] DUAN Xiaowei;ZHANG Ning;WANG Jing;GAO Liwei;LIU Xiujie;WANG Xiwang;YU Guoyuan(Second Department of Neurosurgery,Handan Central Hospital,Handan,Hebei 056000,China)

机构地区：[1]邯郸市中心医院神经外二科,河北邯郸056000

出　　处：《国际检验医学杂志》2024年第12期1463-1468,共6页International Journal of Laboratory Medicine

基　　金：河北省医学科学研究课题计划(20191829)

摘　　要：目的探讨胶质瘤组织长链非编码RNA(lncRNA)小核仁RNA宿主基因(SNHG)25、微小RNA(miR)-497-5p表达与临床特征及预后的关系。方法选择2019年1月至2020年1月该院收治的157例胶质瘤患者为胶质瘤组,同期该院100例因颅脑损伤接受手术治疗的患者为对照组。分别取术中切除的胶质瘤组织和正常脑组织检测lncRNA SNHG25、miR-497-5p表达水平,术后随访3年。分析lncRNA SNHG25表达水平与miR-497-5p的相关性,lncRNA SNHG25、miR-497-5p表达水平与患者临床特征和预后的关系。结果与对照组比较,胶质瘤组lncRNA SNHG25表达水平升高(P<0.05),miR-497-5p表达水平降低(P<0.05)。与肿瘤最大径<4 cm、世界卫生组织(WHO)中枢神经系统肿瘤分级Ⅰ~Ⅱ级比较,肿瘤最大径≥4 cm、WHO中枢神经系统肿瘤分级Ⅲ~Ⅳ级的胶质瘤组织中lncRNA SNHG25表达水平升高,miR-497-5p表达水平降低(P<0.05)。胶质瘤患者的lncRNA SNHG25表达水平与miR-497-5p呈负相关(r=-0.370,P<0.05)。lncRNA SNHG25高表达组累积生存率低于lncRNA SNHG25低表达组(P<0.05),miR-497-5p低表达组累积生存率低于miR-497-5p高表达组(P<0.05)。WHO中枢神经系统肿瘤分级Ⅲ~Ⅳ级、lncRNA SNHG25高表达是胶质瘤患者预后不良的危险因素(P<0.05),miR-497-5p高表达则是保护因素(P<0.05)。结论胶质瘤组织中lncRNA SNHG25表达水平升高,miR-497-5p表达水平降低,且与肿瘤最大径增大、WHO中枢神经系统肿瘤分级高有关,可导致胶质瘤患者不良预后。Objective To investigate the expression of long non-coding RNA(lncRNA)small nucleolar RNA host gene(SNHG)25 and microRNA(miR)-497-5p in glioma tissues and their relationship with clinical features and prognosis.Methods A total of 157 glioma patients admitted to the hospital from January 2019 to January 2020 were selected as the glioma group,and 100 patients who underwent surgical treatment due to craniocerebral injury in the same hospital during the same period were selected as the control group.The expression levels of lncRNA SNHG25 and miR-497-5p were detected in glioma tissues and normal brain tissues resected during operation.The patients were followed up for 3 years.The correlation between the expression levels of lncRNA SNHG25 and miR-497-5p was analyzed,and the relationship between the expression level of lncRNA SNHG25 and miR-497-5p and the clinical characteristics and prognosis of patients were analyzed.Results Compared with the control group,the expression level of lncRNA SNHG25 in the glioma group was increased(P<0.05),and the expression level of miR-497-5p was decreased(P<0.05).Compared with the maximum diameter of tumors<4 cm,World Health Organization(WHO)central nervous system tumor gradeⅠ-Ⅱ,the expression level of lncRNA SNHG25 was increased and the expression level of miR-497-5p was decreased in glioma tissues with the maximum diameter of tumors≥4 cm and WHO central nervous system tumor gradeⅢ-Ⅳ(P<0.05).The expression level of lncRNA SNHG25 in glioma patients was negatively correlated with miR-497-5p(r=-0.370,P<0.05).The cumulative survival rate of lncRNA SNHG25 high expression group was lower than that of lncRNA SNHG25 low expression group(P<0.05),and the cumulative survival rate of miR-497-5p low expression group was lower than that of miR-497-5p high expression group(P<0.05).GradeⅢ-Ⅳof WHO central nervous system tumor grade and high expression of lncRNA SNHG25 were risk factors for poor prognosis of glioma patients(P<0.05),while high expression of miR-497-5p was a protective facto

关 键 词：胶质瘤 长链非编码RNA 小核仁核糖核酸宿主基因 微小RNA-497-5p 

分 类 号：R739.41[医药卫生—肿瘤]