紫檀芪对小鼠缺血性脑损伤后脑水肿期神经细胞凋亡的影响  

The Effect of Pterostilbene on Neuronal Apoptosis during Brain Edema after Ischemia Reperfusion Injury in Mice

作　　者：田士来 康军林 徐小峰 郑茂华[1] 杨刚[1] TIAN Shi-lai;KANG Jun-lin;XU Xiao-feng;ZHENG Mao-hua;YANG Gang(Department of Neurosurgery of the First Hospital of Lanzhou University,Lanzhou,Gansu,730000,China)

机构地区：[1]兰州大学第一医院神经外科,甘肃兰州730000

出　　处：《现代生物医学进展》2024年第10期1813-1817,1872,共6页Progress in Modern Biomedicine

基　　金：甘肃省青年科技基金项目(23JRRA1611);兰州大学第一医院院内基金项目(ldyyyn2020-48)。

摘　　要：目的:探索紫檀芪(PTE)对小鼠缺血性脑损伤后脑水肿期神经细胞凋亡的影响。方法:将实验小鼠分为3组即假手术组(sham组)、脑缺血再灌注损伤组(IR组)和紫檀芪治疗组(PTE+IR组),其中PTE于造模前连续5天每天腹腔给药(5 mg/kg)1次;然后于造模后3 d进行脑组织TTC染色并计算脑梗死体积比;于造模后2 h、12 h和1、2、4、6、8、10、12及14 d进行小鼠神经行为学评分;使用TUNEL试剂盒于造模后3、7和14 d检测缺血半暗带和海马的凋亡神经细胞。结果:PTE可减轻脑梗死体积、改善神经行为学评分以及抑制缺血半暗带和海马的神经细胞凋亡。结论:PTE在小鼠缺血性脑损伤后脑水肿期具有明确的神经保护作用,其机制与抑制细胞凋亡有关。Objective:To explore the effect of pterostilbene on neuronal apoptosis during brain edema after ischemia reperfusion injury in mice.Methods:The experimental mice were randomly divided into three groups:sham group,IR group and PTE+IR group.The dose of PTE was injected intraperitoneally with 5 mg/kg once a day for 5 days totally.The cerebral infarct image was stained by TTC and the cerebral infarct volume was measured by Image J after IR 3 d.The neurological behavioral scores were tested after IR1/12,1/2,1,2,4,6,8,10,12 and 14 d.The neuronal apoptotic percentage of cerebral ischemic penumbra and ipsilateral hippocampus were detected by TUNEL detection kit after IR3,7 and 14 d.Results:PTE lowered cerebral infarct volume,improved neurological behavioral scores and inhibited neuronal apoptosis of cerebral ischemic penumbra and ipsilateral hippocampus.Conclusions:PTE presents a continuous neuroprotective effect against ischemia reperfusion during cerebral edema in mice by suppression of cell apoptosis.

关 键 词：紫檀芪 脑缺血再灌注损伤 细胞凋亡 小鼠 

分 类 号：R-33[医药卫生] R743R965