TRIP13促进子宫内膜癌增殖、迁移和侵袭的影响及作用机制  

Effect and mechanism of TRIP13 on promoting proliferation,migration and invasion in endometrial carcinoma

作　　者：张悦 彭娟[1] 王鲁文 Zhang Yue;Peng Juan;Wang Lu-wen(Department of Obstetrics and Gynecology,The Third Affiliated Hospital of Zhengzhou University,Zhengzhou 450000,China)

机构地区：[1]郑州大学第三附属医院妇产科,河南郑州450000

出　　处：《四川生理科学杂志》2024年第6期1181-1186,共6页Sichuan Journal of Physiological Sciences

摘　　要：目的:探讨甲状腺激素受体因子13(Thyroid hormone receptor interactor 13,TRIP13)对子宫内膜癌(Endometrial carcinoma,EC)增殖、迁移和侵袭的影响及调控机制。方法:分析TRIP13在子宫内膜癌中的表达模式;通过慢病毒敲减和质粒过表达调控子宫内膜癌细胞中TRIP13的表达量;通过CCK-8、Transwell迁移及侵袭实验观察TRIP13对子宫内膜癌细胞增殖、迁移及侵袭能力的影响;通过Western blot技术检测TRIP13表达水平的变化,并研究其对PI3K/Akt信号通路蛋白的影响。结果:癌症基因组图谱(The Cancer Genome Atlas,TCGA)分析发现,子宫内膜癌组织中TRIP13的表达水平显著高于正常子宫内膜组织(P<0.05);TRIP13的高表达与子宫内膜癌患者的低生存率呈显著正相关(P<0.05)。TRIP13过表达可促进子宫内膜癌细胞的增殖、迁移及侵袭能力(P<0.05),并上调p-PI3K、p-Akt的表达(P<0.05),而下调TRIP13后则可逆转上述结果。结论:TRIP13通过调控PI3K/Akt信号通路促进子宫内膜癌细胞的增殖、迁移和侵袭。Objective:To investigate the effect of thyroid hormone receptor interactor 13(TRIP13)on the proliferation,migration and invasion of Endometrial Carcinoma(EC)and its regulatory mechanism.Methods:The expression pattern of TRIP13 in endometrial cancer were analyzed.The modulation of TRIP13 expression in endometrial cancer cells was achieved through lentiviral knockout and plasmid overexpression.CCK-8 assays,Transwell migration,and invasion experiments were conducted to assess the influence of TRIP13 on the proliferation,migration,and invasion of endometrial cancer cells.Western blot analysis was employed to evaluate the impact of TRIP13 expression on proteins in the PI3K/Akt signaling pathway.Results:Analysis of the TCGA database revealed a significantly higher expression level of TRIP13 in endometrial cancer tissues compared to normal endometrial tissues(P<0.05).High TRIP13 expression was significantly and positively associated with a lower survival rate among patients with endometrial cancer(P<0.001).Elevated TRIP13 expression was found to accelerate the proliferation,migration,and invasion of endometrial cancer cells(P<0.01)and was linked to a significant upregulation in the expression of p-PI3K and p-Akt(P<0.05).Conversely,downregulating TRIP13 reversed these outcomes.Conclusion:TRIP13 regulates the PI3K/Akt signaling pathway to facilitate the proliferation,migration and invasion of endometrial cancer.

关 键 词：TRIP13 子宫内膜癌 增殖 迁移 侵袭 PI3K/AKT信号通路 

分 类 号：R737.33[医药卫生—肿瘤]