LncRNA HULC在乳头状甲状腺癌组织中的表达及意义  

The Expression and Significance of LncRNA HULC in Papillary Thyroid Carcinoma Tissues

作　　者：贾淑蔓 王慧慧 李湘汝 JIA Shuman;WANG Huihui;LI Xiangru(The First Affiliated Hospital of Zhengzhou University,Zhengzhou,450000)

机构地区：[1]郑州大学第一附属医院,450000

出　　处：《实用癌症杂志》2024年第6期887-890,共4页The Practical Journal of Cancer

摘　　要：目的探讨长链非编码RNA(lncRNA)肝癌高表达转录本(HULC)在乳头状甲状腺癌组织中的表达及意义。方法选取90例乳头状甲状腺癌(PTC)患者作为研究对象,手术过程中采集PTC组织和距肿瘤边缘2 cm以上的癌旁组织,通过实时荧光定量聚合酶链反应(RT-qPCR)检测HULC相对表达量。将不同序列转染TPC-1细胞,依据转染序列分为Si-HULC组、Si-Con组、空白组,分别对三组的HULC表达量、细胞增殖活性和侵袭能力进行检测。结果PTC组织的HULC相对表达量明显高于癌旁组织(P<0.05)。肿瘤直径≤2 cm、TNM分期Ⅲ~Ⅳ期、有淋巴结转移的PTC癌组织HULC相对表达量明显高于肿瘤直径>2 cm、TNM分期Ⅰ~Ⅱ期和无淋巴结转移者(P<0.05)。与Si-HULC组比较,Si-Con组和空白组24、48、72、96 h的A值明显更高(P<0.05)。与Si-HULC组比较,Si-Con组和空白组HULC相对表达量明显更高,侵袭细胞数量明显更多(P<0.05)。结论HULC在PTC组织中相对表达量较高,且与肿瘤大小、TNM分期、淋巴结转移存在密切关联,只有尽早调节HULC,才能有效降低TOC-1细胞的增殖活性和侵袭能力。Objective To investigate the expression and significance of long chain non coding RNA(lncRNA)and high expression transcript of liver cancer(HULC)in papillary thyroid cancer tissue.Methods 90 patients with papillary thyroid carcinoma(PTC)were selected as the study subjects.During the surgery,PTC tissue and adjacent cancerous tissue were collected at a distance of more than 2cm from the tumor edge.The relative expression level of HULC was detected by real-time fluorescence quantitative polymerase chain reaction(RT qPCR).Transfect TPC-1 cells with different sequences and divide them into Si HULC group,Si Con group,and blank group based on the transfection sequence.The HULC expression level,cell proliferation activity,and invasion ability of the 3 groups were detected.Results The relative expression level of HULC in PTC tissue was significantly higher than that in adjacent tissues(P<0.05).The relative expression level of HULC in PTC cancer tissues with tumor diameter≤2 cm,TNM stageⅢ~Ⅳ,and lymph node metastasis was significantly higher than those with tumor diameter>2 cm,TNM stageⅠ~Ⅱ,and no lymph node metastasis(P<0.05).Compared with the Si HULC group,the A values of the Si Con group and the blank group at 24,48,72,and 96 hours were significantly higher(P<0.05).Compared with the Si HULC group,the Si Con group and the blank group had significantly higher relative expression levels of HULC and a significantly higher number of invasive cells(P<0.05).Conclusion The relative expression of HULC in PTC tissues is high,and it is closely related to tumor size,TNM stage and lymph node metastasis.Only by regulating HULC as early as possible can the proliferation activity and invasion ability of TOC-1 cells be effectively reduced.

关 键 词：长链非编码RNA 肝癌高表达转录本 乳头状甲状腺癌 

分 类 号：R736.1[医药卫生—肿瘤]