miR-223-3p与ECT2表达在食管鳞状细胞癌组织中的相关性及与其预后的关系  

Correlation Between miR-223-3p and ECT2 in Esophageal Squamous Cell Carcinoma and Its Effect on Prognosis

作　　者：范俊利[1] 雷彩鹏 张卫国[1] 赵安顺 FAN Junli;LEI Caipeng;ZHANG Weiguo(The First Affiliated Hospital of Henan University of Science and Technology,Luoyang,471000)

机构地区：[1]河南科技大学第一附属医院,471000

出　　处：《实用癌症杂志》2024年第6期898-903,924,共7页The Practical Journal of Cancer

摘　　要：目的探讨miR-223-3p与ECT2表达在食管鳞状细胞癌中的相关性及与其预后的关系。方法纳入85例食管鳞状细胞癌的石蜡包埋食管鳞状细胞癌组织、相应的正常食管黏膜组织。实时荧光定量PCR检测miR-223-3p与ECT2 mRNA水平,Pearson相关系数分析miR-223-3p与ECT2 mRNA表达水平的相关性。Kaplan-Meier生存分析和Cox比例风险模型分析miR-223-3p、ECT2表达对食管鳞状细胞癌预后的影响。结果ECT2高表达食管鳞状细胞癌患者46例,ECT2低表达食管鳞状细胞癌患者39例。ECT2 mRNA表达水平食管鳞状细胞癌组织高于正常食管黏膜组织,miR-223-3p表达水平食管鳞状细胞癌组织低于正常食管黏膜组织。ECT2表达水平与肿瘤大小、分化程度、TNM分期、血管侵犯相关。miR-223-3p表达水平与肿瘤大小、分化程度、TNM分期、血管侵犯相关。此外,食管鳞状细胞癌中miR-223-3p与ECT2 mRNA表达存在相关性(ρ=-0.5223,P<0.0001),miR-223-3p与ECT2蛋白表达存在相关性(χ^(2)=21.56,P<0.0001)。Kaplan-Meier生存分析显示:TNM分期、淋巴结转移、神经侵犯、miR-223-3p表达水平、ECT2表达水平对食管鳞状细胞癌患者的总生存期有影响。Cox比例风险模型发现TNM分期、淋巴结转移、神经侵犯、miR-223-3p表达水平、ECT2表达水平对总生存期有影响。结论miR-223-3p与ECT2的表达水平在食管鳞状细胞癌中存在相关性。TNM分期、淋巴结转移、神经侵犯、miR-223-3p表达水平、ECT2表达水平可作为ESCC的独立预后因素。Objective To investigate the correlation between miR-223-3p expression and ECT2 expression in esophageal squamous cell carcinoma and its impact on prognosis.Methods Inclusion of 85 cases of esophageal squamous cell carcinoma with paraffin-embedded tissues from esophageal squamous cell carcinoma and corresponding normal esophageal mucosa tissues.Real-time fluorescence quantitative PCR was used to detect the levels of miR-223-3p and ECT2 mRNA.Pearson correlation coefficient analysis was performed to assess the correlation between miR-223-3p and ECT2 mRNA expression levels.Kaplan-Meier survival analysis and Cox proportional hazards model were employed to analyze the impact of miR-223-3p and ECT2 expression on the prognosis of esophageal squamous cell carcinoma.Results T High expression of ECT2 was observed in 46 cases of esophageal squamous cell carcinoma,while 39 cases showed low expression of ECT2.The mRNA expression level of ECT2 in esophageal squamous cell carcinoma tissues was higher than that in normal esophageal mucosa tissues,whereas the expression level of miR-223-3p in esophageal squamous cell carcinoma tissues was lower than that in normal esophageal mucosa tissues.The expression levels of ECT2 were correlated with tumor size,differentiation degree,TNM staging,and vascular invasion.Similarly,the expression levels of miR-223-3p were correlated with tumor size,differentiation degree,TNM staging,and vascular invasion.Additionally,there was a correlation between miR-223-3p and ECT2 mRNA expression in esophageal squamous cell carcinoma(ρ=-0.5223,P<0.0001),as well as between miR-223-3p and ECT2 protein expression(χ^(2)=21.56,P<0.0001).Kaplan-Meier survival analysis revealed that TNM staging,lymph node metastasis,neural invasion,miR-223-3p expression level,and ECT2 expression level had an impact on the overall survival of esophageal squamous cell carcinoma patients.The Cox proportional hazards model further indicated that TNM staging,lymph node metastasis,neural invasion,miR-223-3p expression level,and ECT2 ex

关 键 词：miR-223-3p ECT2 食管鳞状细胞癌 相关性 预后 

分 类 号：R735.1[医药卫生—肿瘤]