当归肉苁蓉纤维复方的润肠通便药效及机制探索  

Efficacy and mechanisms of an Angelica sinensis Cistanche Fiber Compound for constipation relief

作　　者：刘洋 石雅丽[1,2,3,4,5] 吴燕萍 罗祥 梁磊[1,3,4,5] 何蓉蓉 LIU Yang;SHI Ya-li;WU Yan-ping;LUO Xiang;LIANG Lei;HE Rong-rong(College of Pharmacy,Jinan University,Guangzhou 510632,China;School of Chinese Materia Medica,Yunnan University of Chinese Medicine,Kunming 650500,China;Guangdong Engineering Research Center of Chinese Medicine&Disease Susceptibility,Guangdong-Hong Kong-Macao University Joint Laboratory of Traditional Chinese Medicine Internationalization,Jinan University,Guangzhou 510632,China;Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research,College of Pharmacy,Jinan University,Guangzhou 510632,China;International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Chinese Ministry of Education(MOE),Jinan University,Guangzhou 510632,China)

机构地区：[1]暨南大学药学院,广东广州510632 [2]云南中医药大学中药学院,云南昆明650500 [3]暨南大学,广东省疾病易感性及中医药研发工程技术研究中心,中医药国际化研究粤港澳高校联合实验室,广东广州510632 [4]暨南大学药学院,广东省中药药效物质基础及创新药物研究重点实验室,广东广州510632 [5]暨南大学,教育部中药与创新药物研究国际合作联合实验室,广东广州510632

出　　处：《药学学报》2024年第5期1238-1244,共7页Acta Pharmaceutica Sinica

基　　金：广东省粤港澳高校联合实验室(2023LSYS002);广东创新团队(2020KCXTD003)。

摘　　要：便秘是一种常见的疾病,给患者的生活造成很大影响,同时可能并发许多高危因素。本研究使用盐酸洛哌丁胺建立慢性便秘小鼠模型,给予当归肉苁蓉纤维(当归、肉苁蓉、小麦纤维和低聚木糖)复方高(3.6 g·kg-1,30倍人用推荐剂量)、低(0.6 g·kg-1,5倍人用推荐剂量)剂量干预14天,通过观察粪便形态,测量含水率以及小肠运动实验考察复方通便的药效;通过酶联免疫吸附实验(enzyme-linked immunosorbent assay,ELISA)检测小鼠血清中胃肠激素胃动素(motilin,MTL)、胃泌素(gastrin,GAS)以及炎症因子白介素1β(interleukin-1β,IL-1β)、肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)的水平;采用苏木精-伊红(hematoxylin-eosin,H&E)染色检测结肠组织病理损伤;采用免疫蛋白印迹实验(Western blot)和免疫组织化学实验检测结肠中水通道蛋白3(aquaporin 3,AQP3)和c-Kit的表达水平,初步探索相关机制。实验结果显示,给予当归肉苁蓉纤维复方干预后,小鼠粪便形态改善,含水率增加,小肠推进率提高,血清中胃肠激素水平显著升高,炎症因子水平显著降低,结肠病理损伤得到改善,结肠中水通道蛋白AQP3表达水平明显降低,c-Kit表达水平明显升高,结果显示一定量-效关系。说明该当归肉苁蓉纤维复方能够显著改善小鼠便秘,调节血清中炎症因子和胃肠激素水平,改善肠道损伤,且这一作用与调控肠道水通道蛋白AQP3和c-Kit表达有关。本实验获暨南大学动物实验伦理委员会批准。Constipation is a prevalent ailment which might significantly impact the quality of people's life and rise some associated deseases risks.In this study,a chronic constipation mouse model was established using loperamide hydrochloride.Mice were gavaged an Angelica sinensis Cistanche Fiber Compound,comprised of Dang Gui[Angelica sinensis(Oliv.)Diels],Rou Cong Rong(Cistanche deserticola Y.C.Ma),wheat fiber,and lowmolecular-weight xylan at high dosage(3.6 g·kg-1,30 times the recommended human dose)and low dosage(0.6 g·kg-1,5 times the recommended human dose)for 14 days.The study assessed the therapeutic efficacy of the compound by observing fecal morphology,measuring water content,and conducting small intestine motility experiments.Furthermore,enzyme-linked immunosorbent assays(ELISA)were conducted to evaluate the serum levels of gastrointestinal hormones including motilin(MTL),gastrin(GAS),the inflammatory factors interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α).Histopathological examination with H&E staining was employed to evaluate colonic tissue damage.Additionally,Western blot and immunohistochemistry experiments were conducted to examine the expression levels of aquaporin-3(AQP3)and c-Kit in the colon.The results indicated that the Angelica sinensis Cistanche Fiber Compound could improve fecal morphology,increase fecal water content,enhance small intestinal transit rates in mice.Additionally,there was a significant elevation in the serum levels of gastrointestinal hormones and a notable reduction in the levels of inflammatory factors.The improvement in colonic histopathological damage was accompanied by a marked decrease in the expression of the colonic water channel protein AQP3 and a significant increase in c-Kit expression.These results collectively suggested the presence of a dose-response relationship.These findings indicate that the Angelica sinensis Cistanche Fiber Compound effectively alleviates constipation in mice.Its action is associated with the regulation of colonic water channel prote

关 键 词：便秘 润肠通便 功能性食品 结肠 中药 

分 类 号：R966[医药卫生—药理学]