Involvement of 2'-5'oligoadenylate synthetase-like proteinin the survivalof Mycobacterium tuberculosis avirulent strain in macrophages  

作　　者：Aikebaier Reheman Xiaojian Cao Yifan Wang Xi Nie Gang Cao Wei Zhou Bing Yang Yingying Lei Weipan Zhang Muhammad Ahsan Naeem Xi Chen 

机构地区：[1]State Key Laboratory of Agricultural Microbiology,College of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,China [2]College of Animal Science and Technology,Tarim University,Alar 843300,China [3]Bio-Medical Center,Huazhong Agricultural University,Wuhan 430070,China [4]Department of Basic Sciences(Pharmacology),University of Veterinary and Animal Sciences(Narowal Campus),Lahore 54000,Pakistan

出　　处：《Animal Diseases》2023年第4期275-285,共11页动物疾病（英文）

基　　金：funded by the National Natural Science Foundation of China(Grant No.31602061,U21A20259,31872470);the National Key Research and Development Program of China(Grant No.2021YFD1800401).

摘　　要：Mycobacterium tuberculosis(M.tuberculosis)can replicate in the macrophage by interfering with many host protein functions.While it is far from known these host proteins for controlling M.tuberculosis infection.Herein,we infected macrophages including THP-1 and Raw264.7 cells with M.tuberculosis and identified the differentially expressed genes(DEGs)in the interferon signaling pathway.Among them,2'-5'oligoadenylate synthetase-like(OASL)underwent the greatest upregulation in M.tuberculosis-infected macrophages.Knockdown of the expression of OASL attenuated M.tuberculosis survival in macrophages.Further,bioinformatics analysis revealed the potential interaction axis of OASL-TAB3-RvO127,which was further validated by the yeast-two-hybrid(Y2H)assay and Co-IP.This interaction axis might regulate the M.tuberculosis survival and proliferation in macrophages.The study reveals a possible role of OASL during M.tuberculosis infection as a target to control its propagation.

关 键 词：Mycobacterium tuberculosis INTERFERON Interferon stimulated genes OASL 

分 类 号：S85[农业科学—兽医学]