Artificial cavernosa-like tissue based on multibubble Matrigel and a human corpus cavernous fibroblast scaffold  

作　　者：Yu-Zhuo Chen Yi-Hong Zhou Min-Bo Yan Ming Xiao Biao Liu Ying-Hao Yin Xiao-Li Tan Yong-Quan Huang Yu-Hong Lin Ting Xie Jia-Li Tian Qi Wang Jian-Ying Li Zi-Zhou Meng Zheng Li Emily Xing Yu-Xin Tang Ya-Wei Li Zhong-Zhen Su Liang-Yu Zhao 

机构地区：[1]Department of Ultrasound,The Fifth Affiliated Hospital,Sun Yat-sen University,Zhuhai 519000,China [2]Department of Interventional Medicine,Guangdong Provincial Key Laboratory of Biomedical Imaging,The Fifth Affiliated Hospital,Sun Yat-sen University,Zhuhai 519000,China [3]Department of Urology,The Fifth Affiliated Hospital,Sun Yat-sen University,Zhuhai 519000,China [4]Department of Andrology,the Center for Men’s Health,Urologic Medical Center,Shanghai Key Laboratory of ReproductiveMedicine,Shanghai General Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200080,China [5]Cedars-Sinai Medical Center,Los Angeles,CA 90024,USA

出　　处：《Asian Journal of Andrology》2024年第3期260-267,共8页亚洲男性学杂志（英文版）

基　　金：supported by GuangDong Basic and Applied Basic ResearchFoundation(2021A1515111109 to LYZ);the National Natural ScienceFoundation of China(82371632 to YXT);China Postdoctoral ScienceFoundation(2021M703747 to LYZ).

摘　　要：Ex vivo tissue culture of the human corpus cavernosum(cc)can be used to explore the tissue structural changes and complex signaling networks.At present,artificial Cc-like tissues based on acellular or three-dimensional(3D)-printed scaffolds are used to solve the scarcity of primary penis tissue samples.However,inconvenience and high costs limit the wide application of such methods.Here,we describe a simple,fast,and economical method of constructing artificial CC-like tissue.Human Cc fibroblasts(FBs),endothelial cells(ECs),and smooth muscle cells(SMCs)were expanded in vitro and mixed with Matrigel in specific proportions.A large number of bubbles were formed in the mixture by vortexing combined with pipette blowing,creating a porous,spongy,and spatial structure.The Cc FBs produced a variety of signaling factors,showed multidirectional differentiation potential,and grew in a 3D grid in Matrigel,which is necessary for CC-like tissue to maintain a porous structure as a cell scaffold.Within the C-like tissue,ECs covered the surface of the lumen,and SMCs were located inside the trabeculae,similar to the structure of the primary Cc.Various cell components remained stable for 3 days in vitro,but the EC content decreased on the 7th day.Wingless/integrated(WNT)signaling activation led to lumen atrophy and increased tissue fibrosis in CC-like tissue,inducing the same changes in characteristics as in the primary Cc.This study describes a preparation method for human artificial cC-like tissue that may provide an improved experimental platform for exploring the function and structure of the cC and conducting drug screening for erectile dysfunction therapy.

关 键 词：artificial tissue corpus cavernosum ex vivo FIBROBLASTS 

分 类 号：R697[医药卫生—泌尿科学]