基于氨基酸成分的淡水珍珠及海水珍珠对比分析及近红外快速检测  

Comparative analysis and near infrared rapid detection of freshwater Margaritaand seawater Margarita based on amino acid composition

作　　者：张紫涵 王香颖 李思维 李启龙 甘柳霜 郝二伟 杜正彩 侯媛媛 白钢 邓家刚 侯小涛 ZHANG Zihan;WANG Xiangying;LI Siwei;LI Qilong;GAN Liushuang;HAO Erwei;DU Zhengcai;HOU Yuanyuan;BAI Gang;DENG Jiagang;HOU Xiaotao(Collegeof Pharmacy,Guangxi University of Chinese Medicine,Nanning 530200,China;Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica,Guangxi University of Chinese Medicine,Nanning 530200,China;Guangxi Collaborative Innovation Center for Research on Functional lngredients of Agricultural Residues,Nanning 530200,China;Guangxi Key Laboratory of TCM Formulas Theory and Transformation for Damp Diseases,Nanning 530200,China;Tianjin Key Laboratory of Molecular Drug Research,College of Pharmacy,Nankai University,Tianjin 300071,China)

机构地区：[1]广西中医药大学药学院,广西南宁530200 [2]广西中医药大学广西中药药效研究重点实验室,广西南宁530200 [3]农作物废弃物功能成分研究协同创新中心,广西南宁530200 [4]广西中医湿病方药理论与转化重点实验室,广西南宁530200 [5]南开大学药学院天津市分子药物研究重点实验室,天津300071

出　　处：《中草药》2024年第10期3477-3486,共10页Chinese Traditional and Herbal Drugs

基　　金：中国(广西)-东盟药物创新中心建设及新药开发(桂科AA19254033);2023年中央补助国家中医药管理局高水平中医药重点学科建设项目-海洋中药学(桂财社函〔2023〕97号)。

摘　　要：目的基于关键氨基酸的含量差异,通过近红外技术实现淡水与海水珍珠的快速检测。方法采用氨基酸分析仪考察108批次海水珍珠与淡水珍珠氨基酸种类与含量差异,依据氨基酸比较分析筛选关键氨基酸。针对关键氨基酸优化近红外定量模型,确立近红外光谱技术珍珠快速检测模型。结果研究发现天冬氨酸(Asp)和甘氨酸(Gly)含量在淡水珍珠与海水珍珠有显著差异,且两者含量范围无交集。通过珍珠近红外光谱与Asp、Gly含量化学计量学拟合对珍珠样品进行检测,所得结果与传统的氨基酸分析技术测定结果一致。结论基于化学计量学手段对珍珠的定量分析,结果准确可靠,结合近红外光谱技术对不同基原珍珠进行快速检测,为珍珠的质量控制提供方法依据。Objective Based on Based on the difference of the content of key amino acids,the near infrared technology was used to detect the freshwater Margarita and seawater Margarita rapidly.Methods The amino acid analyzer was used to investigate the differences in the types and contents of amino acids between 108 batches of seawater Margarita and freshwater Margarita,and the key amino acids were screened according to the comparative analysis of amino acids.The near-infrared quantitative model was optimized for key amino acids,and the rapid detection model of Margarita was established by near infrared spectroscopy(NIRS).Results The results showed that the contents of aspartic acid(Asp)and glycine(Gly)were significantly different between freshwater Margarita and seawater Margarita,and there were no intersectional content range between Asp and Gly.The NIRS of Margarita was fitted with the stoichiometry of Asp and Gly content to detect Margarita samples,and the obtained results showed consistently with the determination results of traditional amino acid analysis technology.Conclusion The quantitative analysis of Margaritas based on stoichiometry showed that the results were accurate and reliable.The rapid detection of Margarita with different primordia was carried out combined with NIRS,providing a method basis for quality control of Margarita.

关 键 词：珍珠 氨基酸 定量测定 近红外光谱技术 快速检测 

分 类 号：R286.2[医药卫生—中药学]