不同水文情景下白洋淀水体好氧反硝化菌群对水质因子的动态响应  

Dynamic Response of Aerobic Denitrification Bacteria to Water Quality in BaiyangdianLake Under Different Hydrological Scenarios

作　　者：孟佳靖 张甜娜 陈哲 周石磊 底怡玲 武辰彬 王晨光 张家丰 崔建升[1] MENG Jia-jing;ZHANG Tian-na;CHEN Zhe;ZHOU Shi-lei;DI Yi-ling;WU Chen-bin;WANG Chen-guang;ZHANG Jia-feng;CUI Jian-sheng(Hebei Key Laboratory of Pollution Prevention Biotechnology,College of Environmental Science and Engineering,Hebei University of Science and Technology,Shijiazhuang 050018,China)

机构地区：[1]河北科技大学环境科学与工程学院,河北省污染防治生物技术实验室,石家庄050018

出　　处：《环境科学》2024年第6期3627-3637,共11页Environmental Science

基　　金：国家自然科学基金项目(51909056);河北省自然科学基金项目(E2021208011);河北省研究生创新能力培养项目(CXZZSS2023090)。

摘　　要：为探究不同水文情景下白洋淀水体好氧反硝化菌群的演变规律和驱动机制,基于水质调查和高通量测序技术,进行了水体水质因子分析、好氧反硝化菌群α多样性分析、物种组成和网络分析.结果表明,白洋淀水体呈弱碱性,丰水期水体T最高,DO最低,冰封期T最低,DO最高.白洋淀水体在枯水期、丰水期、平水期和冰封期下NH_(4)^(+)-N、NO_(2)^(-)-N、NO_(3)^(-)-N、TN、高锰酸盐指数、Fe和Mn之间均存在显著差异(P<0.01),其中TP在不同水文情景下不存在显著差异(P>0.05).不同水文情景下水体中最大的细菌门类为Proteobacteria,相对丰度较高的属为Magnetospirillum、Aeromonas、Pseudomonas、Azospirillum和Bradyrhizobium.此外,好氧反硝化菌群的α多样性均存在显著差异(P<0.001),冰封期菌群丰富度最高,枯水期和冰封期菌群的多样性和均匀度最高.经RDA、Mantel分析,菌群的水质驱动因子在不同水文情景下有所差异,枯水期菌群的水质驱动因子为p H、NO_(3)^(-)-N、NO_(2)^(-)-N和高锰酸盐指数,丰水期菌群的驱动因子为p H、T、DO、NO_(2)^(-)-N和TP,平水期的驱动因子为NO_(2)^(-)-N、Fe和高锰酸盐指数,冰封期菌群的驱动因子为NO_(3)^(-)-N和NO_(2)^(-)-N.网络分析表明与水质驱动因子相关的物种存在时间差异,枯水期与水质驱动因子相关的属为Magnetospirillum、Aeromonas和Azoarcus,与丰水期相关的属为Magnetospirillum、Pseudomonas和Aeromonas,与平水期相关的属为Magnetospirillum、Pseudomonas和Limnohabitans,与冰封期相关的属为Magnetospirillum、Azoarcus和Pseudomonas.不同水文情景关键水质因子(主要是T、DO、NO_(3)^(-)-N和高锰酸盐指数)与好氧反硝化菌群之间的关联关系,随着时间演变也在逐渐发生变化.综上,通过对不同水文情景下白洋淀水体好氧反硝化菌群演变特征及环境因子驱动机制进行研究,可为认识天然环境中好氧反硝化菌群演变机制提供依据.In order to explore the evolution law and driving mechanism of aerobic denitrification bacteria in Baiyangdian Lake under different hydrological scenarios,based on water quality survey and high-throughput sequencing technology,this study conducted a water quality factor analysis and aerobic denitrification bacteriaα-diversity analysis,species composition,and network analysis.The results showed that the water body of Baiyangdian Lake was weakly alkaline,with the highest T and the lowest DO in the rainy season and the lowest T and the highest DO in the freezing season.There were significant differences between NH_(4)^(+)-N,NO_(2)^(-)N,NO_(3)^(-)N,TN,permanganate index,Fe,and Mn in Baiyangdian water under different hydrological scenarios(P<0.01),and there was no significant difference in TP under different hydrological scenarios(P>0.05).The largest category in water bodies under different hydrological scenarios was Proteobacteria,and the genera with a higher relative abundance were Magnetospirillum,Aeromonas,Pseudomonas,Azospirillum,and Bradyrhizobium.In addition,within the aerobic denitrifying bacteria community,there were significant differences inα-diversity(P<0.001),with the highest abundance of microbial communities occurring during the freezing period,and the highest diversity and evenness of microbial communities during the dry and freezing periods.According to the RDA and Mantel analyses,the water quality driving factors of flora were different under different hydrological scenarios.The water quality driving factors of flora in the dry season were pH,NO_(3)^(-)N,NO_(2)^(-)N,and permanganate index;the driving factors of flora in the rainy season were pH,T,DO,NO_(2)^(-)N,and TP;the driving factors of flora in the normal season were NO_(2)^(-)N,Fe,and permanganate index;and the driving factors of flora in the freezing season were NO_(3)^(-)N and NO_(2)^(-)N.Network analysis showed that there were temporal differences in species related to water quality driving factors.The genera related to water quality drivi

关 键 词：好氧反硝化 白洋淀 水文情景 网络分析 水质因子 

分 类 号：X172[环境科学与工程—环境科学]