结直肠癌中含溴结构域蛋白9的亚细胞定位模式及其临床意义  

Subcellular localization pattern of bromodomain containing 9 in colorectal cancer and its clinical significance

作　　者：刘春铖 刘晓晗 魏闫若雪 李梓绮 刘玉昆 赵然 LIU Chuncheng;LIU Xiaohan;WEI Yanruoxue;LI Ziqi;LIU Yukun;ZHAO Ran(Cheeloo College of Medicine,Shandong University,Jinan 250012,Shandong,China;Department of Pathology,Affiliated Hospital of Jining Medical University,Jining 272029,Shandong,China)

机构地区：[1]山东大学齐鲁医学院,山东济南250012 [2]济宁医学院附属医院病理科,山东济宁272029

出　　处：《山东大学学报（医学版）》2024年第4期24-30,共7页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金(81902815,81802786);山东省自然科学基金(ZR2023MH011,ZR2019BH044,ZR2018BH025)。

摘　　要：目的探讨结直肠癌(colorectal cancer,CRC)中含溴结构域蛋白9(bromodomain containing 9,BRD9)的蛋白表达水平、亚细胞定位模式及其临床意义。方法收集CRC手术切除标本,采用免疫组织化学技术EnVision法检测BRD9的蛋白表达水平及其亚细胞定位,并分析其与患者临床病理特征及预后的关系。结果在508例CRC组织标本中,BRD9高表达患者为71.46%(363/508),BRD9细胞核阳性患者为80.31%(408/508);BRD9的表达与患者肿瘤大小(P=0.025)、淋巴结转移(P=0.004)、神经侵犯(P=0.043)、分化程度(P=0.017)及TNM分期(P=0.002)相关,BRD9的亚细胞定位与患者淋巴结转移(P=0.040)及TNM分期(P=0.026)相关,差异有统计学意义;BRD9高表达可作为患者的独立不良预后因素(HR=1.994,P=0.002),同时,在BRD9低表达患者中,BRD9细胞浆阳性可作为患者的独立不良预后因素(HR=8.056,P=0.001)。结论在CRC患者中BRD9的亚细胞定位模式具有异质性,其高表达及细胞浆定位与CRC的发生发展密切相关,并能够作为独立预测因子预测患者的生存预后。Objective To investigate the protein expression level,subcellular localization pattern and clinical significance of bromodomain containing 9(BRD9)in colorectal cancer(CRC).Methods Surgical resection specimens of CRC were collected,and immunohistochemistry(EnVision method)was used to detect BRD9 protein expression and subcellular localization.The relationship between BRD9 expression and clinicopathological features and prognosis of CRC patients was analyzed.Results Among 508 CRC tissue specimens,BRD9 was overexpressed in 71.46%(363/508)of patients,and 80.31%(408/508)of patients showed nuclear positive staining in immunohistochemistry for BRD9.The expression of BRD9 was correlated with tumor size(P=0.025),lymph node metastasis(P=0.004),nerve invasion(P=0.043),differentiation grade(P=0.017)and TNM stage(P=0.002).The subcellular localization of BRD9 was correlated with lymph node metastasis(P=0.040)and TNM stage(P=0.026).The difference was statistically significant.Meanwhile,BRD9 high expression served as an independent poor prognostic factor(HR=1.994,P=0.002).More important,in patients with BRD9 low expression,cytoplasmic BRD9 expression was an independent poor prognostic factor of patients(HR=8.056,P=0.001).Conclusion The subcellular localization of BRD9 is heterogeneous in CRC patients.The high expression and cytoplasmic localization of BRD9 are related to the occurrence and development of CRC,and could be used as an independent predictor for the prognosis of CRC patients.

关 键 词：结直肠癌 免疫组织化学 含溴结构域蛋白9 亚细胞定位 预后 

分 类 号：R735.3[医药卫生—肿瘤]