山苍子油对猕猴桃溃疡病菌的抑菌活性及应用研究  

Inhibitory Activity and Field Control Effect of Litsea cubeba Oil on Pseudomonas syringae pv.actinidiae

作　　者：颜志豪 董卫国 左仁芳 杨欣朋 李晓刚[1,2] YAN Zhi-hao;DONG Wei-guo;ZUO Ren-fang;YANG Xin-peng;LI Xiao-gang(College of Plant Protection,Hunan Agricultural University,Changsha 410128,PRC;Hunan Provincial Engineering Research Center of Pest Early Warning and Control,Changsha 410128,PRC;Plant Protection and Inspection Station of Luxi County Bureau of Agriculture and Rural Affairs,Luxi 416100,PRC;Plant Protection and Inspection Station of Fenghuang County Bureau of Agriculture and Rural Affairs,Fenghuang 416200,PRC)

机构地区：[1]湖南农业大学植物保护学院,湖南长沙410128 [2]湖南农业有害生物预警与防控工程研究中心,湖南长沙410128 [3]泸溪县农业农村局植保植检站,湖南泸溪416100 [4]凤凰县农业农村局植保植检站,湖南凤凰416200

出　　处：《湖南农业科学》2024年第5期72-78,共7页Hunan Agricultural Sciences

摘　　要：采用室内平板抑制试验评价抑菌效果的方法,检测出山苍子油对猕猴桃溃疡病菌表现明显的抑制作用;对分离纯化的湘西地区猕猴桃溃疡病病原菌分子生物学鉴定为丁香假单胞菌猕猴桃致病变种;通过乳化剂筛选,找到最佳复配乳化剂组合SK-33SC:YUS-D935=1∶1(质量比),并配制出25%山苍子油EW;采用比浊法测定25%山苍子油EW对猕猴桃溃疡病菌的EC_(50)值为8.581mg/L,回归方程为y=-2.21+2.38x,相关系数为0.965;田间试验结果表明,25%山苍子油EW 200倍稀释液对猕猴桃溃疡病秋季防效达到82.69%、春季防效达到78.92%,且较2%春雷霉素水剂(加收米)以400倍液喷雾的秋季防效高60.91%、春季防效高38.30%。The indoor plate inhibition test confirmed the inhibitory effect of Litsea cubeba oil on Pseudomonas syringae pv.actinidiae.The pathogen causing kiwifruit canker in western Hunan was isolated and identified as P.syringae pv.actinidiae based on the molecular evidence.The composition of the emulsifier combination was optimized as SK-33SC∶YUS-D935=1∶1(mass ratio),and L.cubeba oil 25%EW was prepared.The median effective concentration(EC_(50))of L.cubeba oil 25%EW against P.syringae pv.actinidiae was determined as 8.581 mg/L by turbidimetry,with the regression equation of y=-2.21+2.38x(r=0.965).The field experiment results showed that the control effect of the 200×diluent of L.cubeba oil 25%EW on kiwifruit canker reached 82.69%in autumn and 78.92%in spring,which were 60.91%and 38.30%higher than those of 400×diluent of 2%kasugamycin aqueous solution in autumn and in spring,respectively.

关 键 词：猕猴桃 溃疡病 山苍子油 乳化剂 

分 类 号：S436.634[农业科学—农业昆虫与害虫防治]