基于UHPLC-Q-TOF-MS/MS技术分析大花萱草花的化学成分  

Chemical composition analysis of Hemerocallis hybrida Bergmans based on UHPLC-Q-TOF-MS/MS technology

作　　者：孙宇 张金玲 马玉坤[1] 胡乌兰 马天成 Sun Yu;Zhang Jinling;Ma Yukun;Hu Wulan;Ma Tiancheng(School of Pharmacy,Qiqihar Medical University,Qiqihar,Heilongjiang 161006,China)

机构地区：[1]齐齐哈尔医学院药学院,黑龙江齐齐哈尔161006

出　　处：《齐齐哈尔医学院学报》2024年第11期1048-1053,共6页Journal of Qiqihar Medical University

基　　金：黑龙江省教育厅省属本科高等学校基本科研业务费科研项目(2021-KYYWF-0338)。

摘　　要：目的采用超高效液相色谱串联四级杆飞行时间质谱对大花萱草花的化学成分进行分析。方法在负离子模式下,采用Waters HSS T3色谱柱,以0.1%甲酸乙腈(A)-0.1%甲酸水(B)为流动相进行梯度洗脱,流速为0.4 ml/min,柱温为35℃,进样量为4μl。建立萱草属化学成分数据库,通过精确的m/z值、特征二级数据、多种数据库和文献中的裂解途径等对化合物进行鉴定。结果共鉴定出59个化合物,包括25个黄酮类、22个苯丙素类、4个脂肪酸类、2个生物碱类以及6个其他类的化合物,其中12个化合物为首次在萱草属植物中发现。结论本方法首次系统、全面、快速地筛选并鉴别大花萱草花中的化合物,为大花萱草花的开发和深入研究提供基础。Objective By adopting ultra high performance liquid chromatography cascade Quadrupole time-of-flight mass spectrometry to analyze the chemical composition of Hemerocallis hybrida Bergmans.Methods The gradient elution was carried out on A Waters HSS T3 column with 0.1%formic acid acetonitrile(A)-0.1%formic acid water(B)as the mobile phase in negative ion mode.The flow rate was 0.4 ml/min,the column temperature was 35℃,and the injection volume was 4μl.The chemical composition database of Hemerocallis was established,and the compounds were identified by accurate m/z values,characteristic secondary data,a variety of databases and cleavage pathways in the literature.Results A total of 59 compounds were identified,including 25 flavonoids,22 phenylpropanoids,4 fatty acids,2 alkaloids and 6 other compounds.Among them,12 compounds were first reported in Hemerocallis.Conclusions This method is the first time to systematically,comprehensively and quickly screen and identify the compounds in Hemerocallis hybrida Bergmans,which provides a basis for the development and in-depth study of Hemerocallis hybrida Bergmans.

关 键 词：大花萱草花 超高效液相色谱-四极杆-飞行时间串联质谱法 化学成分 

分 类 号：R914[医药卫生—药物化学]