七氟烷预处理对1-甲基-4苯基-1,2,3,4-四氢吡啶诱导的急性帕金森病小鼠的神经保护作用  

Neuroprotective effect of sevoflurane preconditioning on 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine induced acute Parkinson's disease in mice

作　　者：卓明[1] 邓子豪 刘金龙[1] 钟茂林[1] 李盈 管俊辉 李斌[1] 梁伟东 李艺 叶军明[1] ZHUO Ming;DENG Zihao;LIU Jinlong;ZHONG Maolin;LI Ying;GUAN Junhui;LI Bin;LIANG Weidong;LI Yi;YE Junming(Department of Anesthesiology,the First Affiliated Hospital of Gannan Medical University,Ganzhou,Jiangxi,341000,China;The First Clinical Medical College of Gannan Medical University,Ganzhou,Jiangxi,341000,China)

机构地区：[1]赣南医学院第一附属医院麻醉科,江西赣州341000 [2]赣南医学院第一临床医学院,江西赣州341000

出　　处：《当代医学》2024年第7期1-5,共5页Contemporary Medicine

基　　金：江西省卫生健康委科技计划项目(202130623);赣州市卫生健康委员会市级科研计划项目(2020-2-88);江西省中医药管理局科技计划项目(2021B662)。

摘　　要：目的探讨七氟烷预处理对1-甲基-4苯基-1,2,3,4-四氢吡啶(MPTP)诱导的急性帕金森病(PD)小鼠的神经保护作用。方法选取36只SPF级雄性C57BL/6小鼠并随机分为4组,每组9只。七氟烷组暴露于2%七氟烷2h,PD组腹腔注射MPTP20mg/kg,七氟烷预处理+PD组预先暴露于2%七氟烷2h,复苏后于小鼠腹腔注射MPTP,对照组腹腔注射等剂量0.9%氯化钠溶液。比较4组行为学指标、黑质区酪氨酸羟化酶(TH)阳性神经元数量、脑组织炎症因子[白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)]水平、氧化应激指标[丙二醛(MDA)、谷胱甘肽(GSH)]水平。结果PD组、七氟烷预处理+PD组悬挂试验掉落潜伏期均短于对照组、七氟烷组,悬挂试验评分低于对照组、七氟烷组,爬杆试验转向时间、总时间均长于对照组、七氟烷组,且七氟烷预处理+PD组悬挂试验掉落潜伏期长于PD组,悬挂试验评分高于PD组,爬杆试验转向时间、总时间均短于PD组,差异有统计学意义(P<0.05)。PD组、七氟烷预处理+PD组黑质区TH阳性神经元数量均少于对照组、七氟烷组,七氟烷预处理+PD组黑质区TH阳性神经元数量多于PD组,差异有统计学意义(P<0.05)。PD组、七氟烷预处理+PD组脑组织IL-1β、IL-6、TNF-α水平均高于对照组、七氟烷组,七氟烷预处理+PD组脑组织IL-1β、IL-6、TNF-α水平低于PD组,差异有统计学意义(P<0.05)。PD组、七氟烷预处理+PD组脑组织MDA水平均高于对照组、七氟烷组,GSH水平低于对照组、七氟烷组,且七氟烷预处理+PD组脑组织MDA水平低于PD组,GSH水平高于PD组,差异有统计学意义(P<0.05)。结论七氟烷预处理对MPTP诱导的急性PD模型小鼠具有保护作用,可改善运动功能,其作用机制可能与七氟烷预处理的抗炎、抗氧化应激相关。Objective To investigate the neuroprotective effect of sevoflurane preconditioning on 1-methyl-4-phenyl-1,2,3,6-tetrahydropyri-dine(MPTP)induced acute Parkinson's disease(PD)in mice.Methods 36 SPF male C57BL/6 mice were selected and randomly divided into 4 groups,with 9 mice in each group.The sevoflurane group was exposed to 2%sevoflurane for 2 h,the PD group was intraperitoneally injected with MPTP 20 mg/kg,the sevoflurane pretreatment+PD group was pre-exposed to 2%sevoflurane for 2 h,and the mice were intraperitoneally injected with MPTP after resuscitation,the control group was intraperitoneally injected with the equal dose of 0.9%sodium chloride solution.The behavioral indexes,the number of tyrosine hydroxylase(TH)positive neurons in substantia nigra,the levels of inflammatory factors(interleukin-1β[IL-1β],in-terleukin-6[IL-6],tumor necrosis factor-α[TNF-α])and oxidative stress(malondialdehyde[MDA]and glutathione[GSH])in brain tissues were com-pared among the four groups.Results The falling latency of suspension test in the PD group and the sevoflurane pretreatment+PD group was short-er than that in the control group and the sevoflurane group,the suspension test score was lower than that in the control group and the sevoflurane group,the turning time and total time of pole climbing test were longer than those in the control group and the sevoflurane group,and the falling la-tency of suspension test in the sevoflurane pretreatment+PD group was longer than that in the PD group,the suspension test score was higher than that in the PD group,the turning time and total time of pole climbing test were shorter than those in the PD group,the differences were statistically significant(P<0.05).The number of TH positive neurons in substantia nigra in the PD group and the sevoflurane preconditioning+PD group was less than that in the control group and sevoflurane group,and the number of TH positive neurons in substantia nigra in the sevoflurane pretreatment+PD group was more than that in the PD group,the differences w

关 键 词：七氟烷 预处理 帕金森病 炎症反应 氧化应激 

分 类 号：R742.5[医药卫生—神经病学与精神病学]