对羟基苯甲酸丙酯(PrP)对雌性食蚊鱼的组织损伤及相关基因表达的影响  

Tissue Injuries and Related Genes Expression Changes of Female Mosquito-fish(Gambusia affinis)Induced by Propylparaben(PrP)

作　　者：郭美莎 李钰静 梁延鹏 马雲 王海琴 车佳祥 宋晓红[1,2,3] Guo Meisha;Li Yujing;Liang Yanpeng;Ma Yun;Wang Haiqin;Che Jiaxiang;S ong Xiaohong(College of Environmental Science and Engineering,Guilin University of Technology,Guilin 541006,China;The Guangxi Key Laboratory of Theory and Technology for Environmental Pollution Control,Guilin 541006,China;Guangxi Collaborative Innovation Center for Water Pollution Control and Water Safety in Karst Area,Guilin 541006,China)

机构地区：[1]桂林理工大学环境科学与工程学院,桂林541006 [2]广西环境污染控制理论与技术重点实验室,桂林541006 [3]广西岩溶地区水污染控制与用水安全保障协同创新中心,桂林541006

出　　处：《生态毒理学报》2024年第3期373-386,共14页Asian Journal of Ecotoxicology

基　　金：国家自然科学基金项目(42307371);桂林理工大学科研启动金资助项目(GUTQDJJ6614039)。

摘　　要：对羟基苯甲酸丙酯(propylparaben,PrP)作为防腐剂,广泛添加于食品、药品和个人护理品中,其大量使用对水环境构成了潜在威胁。本研究以野生来源的食蚊鱼(Gambusia affinis)雌鱼为研究对象,开展了不同浓度PrP(0.15、6.0和240.0μg·L^(-1))的4 d和16 d暴露实验,以鱼体大脑、鱼鳃和肝脏的组织切片为毒性效应指标,分析了食蚊鱼抗氧化与内分泌功能相关基因mRNA的表达变化。结果表明,不同暴露时间PrP对食蚊鱼的鱼鳃、肝脏组织均造成不同程度的损伤。随着暴露时间延长,大脑的抗氧化相关基因表达量上调,但鱼鳃的抗氧化相关基因和肝脏的cat、cyp4501a基因表达量下调,氧化应激反应逐渐减弱。PrP暴露4 d后,随暴露剂量的增加,大脑组织的内分泌相关基因呈现先升高后下降的趋势;暴露16 d后,大脑组织的内分泌相关基因相对于对照组呈现升高的趋势。PrP暴露4 d后,与对照组相比,肝脏组织的内分泌相关基因中,低浓度处理组(0.15μg·L^(-1)PrP)vtgB基因和高浓度处理组(240.0μg·L^(-1)PrP)的arβ基因表达量有显著差异,其他处理组无显著变化;暴露时间延长至16 d时,各处理组肝脏组织样品的erα、erβ、arα、arβ、vtgC和vtgB基因的表达量上调,表明PrP对食蚊鱼具有雌激素效应。本研究从鱼体组织损伤和关键功能基因mRNA表达变化,揭示PrP对食蚊鱼的毒性损伤、氧化应激和内分泌干扰作用,为PrP的潜在健康风险评估和安全应用提供了科学依据。Propylparaben(PrP)is widely applied in food,pharmaceuticals and personal care products as preservatives,and the extensive usage of PrP has posed potential threat to aquatic ecosystem.In this study,wild-sourced f emale mosquitofish were treated with different concentrations of PrP(0.15,6.0 and 240.0μg·L^(-1))for 4 d and 16 d respectively.The tissue sections as well as mRNA expression changes of antioxidant and endocrine-related genes from the brain,gills and liver,were analyzed to investigate the toxicological effects of PrP on mosquitofish.The r esults showed that different PrP exposure time resulted in varying degrees of damage to the gills and liver of m osquitofish.With the extension of exposure time,the expression of antioxidant-related genes in the brain was u p-regulated,whereas the expression of antioxidant-related genes in the gills,as well as cat and cyp4501a genes in the liver,were down-regulated,and the oxidative stress response in the gills and liver gradually weakened.When f emale mosquitofish were exposed to PrP for 4 d,the endocrine-related genes in the brain increased firstly and then decreased with increasing PrP dose,while in the 16-d-PrP-exposure groups,the genes showed an increased trend compared with that of the control.After 4 d of PrP exposure,a significant difference was observed in the expression of the vtgB gene within liver tissues between the 0.15μg·L^(-1)PrP group and the control group,as well as in the expression of the arβgene within liver tissues between the 240.0μg·L^(-1)PrP group and the control group;however,no significant differences were found in other endocrine-related genes among different PrP groups and the control group.When the exposure time was extended to 16 d,the expression of erα,erβ,arα,arβ,vtgC and vtgB genes in the liver was all up-regulated in the three treatment groups,indicating that PrP had estrogenic effects on mosquitofish.This study revealed the toxic damage,oxidative stress and endocrine disrupting effects of PrP on mosquitofish,through the pheno

关 键 词：对羟基苯甲酸丙酯 食蚊鱼 内分泌干扰 雌鱼 组织损伤 

分 类 号：X171.5[环境科学与工程—环境科学]