高危型HPV感染患者阴道微生态特点及miR-155、miR-222表达水平的相关性分析  

Correlation analysis of vaginal microbiota characteristics and miR-155 and miR-222 expression levels in high-risk HPV infection patients

作　　者：鲍亚玲[1] 贾维宁[1] 杨子娇 BAO Yaling;JIA Weining;YANG Zijiao(Zhangjiakou University,Zhangjiakou 075000,Hebei,China;Hebei Province Hospital of Chinese Medicine)

机构地区：[1]张家口学院,河北张家口075000 [2]河北省中医院

出　　处：《中国病原生物学杂志》2024年第6期648-652,共5页Journal of Pathogen Biology

摘　　要：目的探析高危型HPV感染患者感染HPV亚型情况、阴道微生态特点及miR-155、miR-222表达水平的相关性。方法选取2023年妇科接诊的212例宫颈疾病患者为研究对象。采集患者宫颈管内分泌物,检测14种高危型HPV,包括HPV16、18、31、33、35、39、51、52、53、56、58、59、68、82。采集患者阴道壁分泌物标本,分别进行氨气味试验;制作涂片观察菌群密集度、菌群多样性、霉菌、线索细胞、乳酸杆菌等,进行Nugengt评分;检测解脲脲原体、衣原体、滴虫感染情况。采用阴道微生态联合测定试剂盒测定过氧化氢、唾液酸苷酶、白细胞酯酶水平。采用荧光定量PCR法检测患者宫颈脱落细胞中miR-222、miR-155的表达量。采用Spearman相关系数分析法分析高危HPV患者阴道微生态特点与miR-155、miR-222表达水平的相关性。结果212例患者中,85例检出高危型HPV,检出率为40.09%。其中,25例为HPV-16感染,9例为HPV-58感染,HPV-52、HPV-53感染各7例,HPV-35、HPV-39感染各6例,HPV-31、HPV-51感染各5例,HPV-33、HPV-56感染各4例,3例为HPV-18感染,2例为HPV-59感染,HPV-68、HPV-82感染各1例。高危型HPV阳性与高危型HPV阴性患者中,过氧化氢、细菌性阴道病、解脲脲原体、衣原体阳性率及乳酸杆菌异常率差异有统计学意义(P<0.05),唾液酸苷酶、白细胞酯酶、霉菌、滴虫阳性率差异无统计学意义(P>0.05)。高危HPV感染组患者宫颈脱落细胞中miR-155相对表达量为(1.47±0.30),miR-222相对表达量为(3.02±0.47),高危HPV阴性组患者宫颈脱落细胞中miR-155相对表达量为(1.05±0.20),miR-222相对表达量为(1.68±0.37)。两组患者宫颈脱落细胞中miR-155、miR-222相对表达量差异有统计学意义(P<0.05)。宫颈脱落细胞miR-155的表达量与细菌性阴道病阳性率、解脲脲原体阳性率、衣原体阳性率、乳酸杆菌异常率呈正相关,差异有统计学意义(P<0.05)。宫颈脱落细胞miR-222的表达量与过氧�Objective To explore the correlation between HPV subtypes,vaginal microbiota characteristics,and miR-155 and miR-222 expression levels in high-risk HPV infected patients.Methods 212 patients with cervical diseases admitted to the gynecology department in 2023 were selected as the research subjects.The cervical secretions were collected from patients to detect 14 high-risk types of HPV,including HPV16,18,31,33,35,39,51,52,53,56,58,59,68,82.The samples of vaginal wall secretions from patients were collected to conduct ammonia odor tests separately;The smears were made to observe the density and diversity of microbial communities,mold,clue cells,lactobacilli,etc.,and the Nugengt score was performed;The infection status of Ureaplasma,Chlamydia,and Trichomonas were detected.The levels of hydrogen peroxide,sialidase,and leukocyte esterase were measured by a vaginal microbiota combined assay kit.The expression levels of miR-222 and miR-155 in cervical exfoliated cells of patients were detected by fluorescence quantitative PCR.The Spearman correlation coefficient analysis method was used to analyze the correlation between the vaginal microbiota characteristics of high-risk HPV patients and the expression levels of miR-155 and miR-222.Results Among 212 patients,85 cases were detected as high-risk HPV,with a detection rate of 40.09%.Among them,25 cases were HPV-16 infection,9 cases were HPV-58 infection,7 cases were HPV-52 and 53 infection each,6 cases were HPV-35 and 39 infection each,5 cases were HPV-31 and 51 infection each,4 cases were HPV-33 and 56 infection each,3 cases were HPV-18 infection,2 cases were HPV-59 infection,1 case was HPV-68 and 1 case was HPV-82 infection.There was a statistically significant difference in the positive rates of hydrogen peroxide,bacterial vaginosis,Ureaplasma urealyticum,Chlamydia,and abnormal rates of lactobacilli between high-risk HPV positive and high-risk HPV negative patients(P<0.05),while there was no statistically significant difference in the positive rates of sialidase,leukocy

关 键 词：高危型人乳头瘤病毒 阴道微生态 微小RNA-155 微小RNA-222 

分 类 号：R737.33[医药卫生—肿瘤]