人参皂苷Rg3对人乳腺癌细胞的代谢活性及caspase 3、CDK2表达的影响  

Effects of ginsenoside Rg3 on metabolic activity and expression of caspase 3 and CDK2 in human breast cancer cells

作　　者：娄彦文 李涵 李运鸿 徐萌冉 魏洋行 随蓓蓓 Lou Yanwen;Li Han;Li Yunhong;Xu Mengran;Wei Yanghang;Sui Beibei(School of Biological Science,Jining Medical University,Rizhao 276826,China;Department of Emergency,Jining First People's Hospital,Jining 272000,China)

机构地区：[1]济宁医学院生物科学学院,日照276826 [2]济宁市第一人民医院急诊科,272000

出　　处：《中华诊断学电子杂志》2024年第2期90-94,共5页Chinese Journal of Diagnostics(Electronic Edition)

基　　金：山东省中医药科技面上项目(2021M133);济宁医学院大学生创新训练计划项目(cx2022100z)。

摘　　要：目的:探讨人参皂苷Rg3对人乳腺癌细胞MDA-MB-231代谢活性、凋亡和增殖的影响。方法:体外培养人乳腺癌细胞MDA-MB-231,分为对照组(正常培养)和Rg3处理实验组(0.4 mg/ml,0.8 mg/ml,1.6 mg/ml),采用四甲基偶氮唑蓝(MTT)法检测各组细胞24 h内的代谢活性;采用凋亡试剂盒检测各组细胞凋亡因子caspase 3的酶活性;采用电镜观察各组细胞形态学变化;采用蛋白质印迹(Western blot)法检测细胞周期蛋白依赖性激酶2(CDK2)蛋白的表达水平。采用t检验比较两组间caspase 3酶活力和CDK2蛋白表达水平。结果:不同浓度Rg3处理人乳腺癌细胞MDA-MB-231后,细胞活性变弱,具有时间和浓度依赖性。凋亡试剂盒检测发现,Rg3浓度为0.4 mg/ml、0.8 mg/ml、1.6 mg/ml时分别处理细胞24 h后,caspase 3酶活力分别为(116.00±8.71)、(171.00±10.82)、(95.67±5.03),仅Rg3浓度为0.8 mg/ml时,与对照组(105.33±7.51)比较,差异有统计学意义(t=8.64,P<0.01)。电镜观察结果显示,0.4 mg/ml和0.8 mg/ml的Rg3处理实验组细胞出现了凋亡典型特征,1.6 mg/ml Rg3处理实验组细胞出现碎片化。Western blot结果显示,0.8 mg/ml Rg3处理实验组细胞的CDK2蛋白的表达水平(0.76±0.03)与对照组(0.89±0.07)比较显著降低(t=2.84,P<0.05)。结论:浓度为0.8 mg/ml的Rg3可对人乳腺癌细胞MDA-MB-231代谢活性有明显的抑制作用,促使细胞发生凋亡,降低细胞增殖因子CDK2的表达,对细胞增殖有一定的抑制作用。Objective To investigate the effect of ginsenoside Rg3 on the activity,apoptosis and proliferation of human breast cancer cell line MDA-MB-231.Methods Human breast cancer cell line MDA-MB-231 was cultured in vitro and divided into control group(normal culture)and Rg3 treatment group(0.4mg/ml,0.8mg/ml,1.6mg/ml).The metabolic activity of cells in each group within 24h was detected by methyl thiazolyl terazolium(MTT)assay.Apoptosis kit was used to detect the activity of caspase 3 in each group.The morphological changes of cells in each group were observed by electron microscopy.The expression level of cyclin-dependent kinase 2(CDK2)protein was detected by Western blot.Comparison between the enzymatic activity of caspase 3 and the protein expression levels of CDK2 was conducted using t-test.Results Human breast cancer cell line MDA-MB-231 was treated with different concentrations of Rg3,and the cell activity was weakened in a time and dose dependent manner.Upon detection with the apoptosis detection kit,it was found that when the concentrations of Rg3 were 0.4mg/ml,0.8mg/ml,and 1.6mg/ml respectively,the caspase 3 enzyme activities after treating the cells for 24 hours were(116.00±8.71),(171.00±10.82),and(95.67±5.03)respectively.Only at the concentration of 0.8mg/ml of Rg3,there was a statistically significant difference compared to the control group(105.33±7.51)(t=8.64,P<0.01).The results of electron microscopy showed that the experimental group with Rg3 concentration of 0.4mg/ml and 0.8mg/ml showed typical apoptotic characteristics,while the experimental group with Rg3 concentration of 1.6mg/ml showed fragmentation.Western blot results showed that the expression level of CDK2 protein in 0.8mg/ml Rg3 treatment group(0.76±0.03)was significantly decreased compared with that in the control group(0.89±0.07)(t=2.84,P<0.05).Conclusion Rg3 with a concentration of 0.8mg/ml can significantly inhibit the metabolic activity of human breast cancer cell line MDA-MB-231,promote cell apoptosis,reduce the expression of cell pr

关 键 词：人参皂苷RG3 乳腺肿瘤 细胞周期蛋白依赖性激酶2 凋亡 增殖 

分 类 号：R737.9[医药卫生—肿瘤]