Co-immunoprecipitation for identifying protein-protein interaction on lipid droplets  

作　　者：Xiaochuan Fu Shuyan Zhang Pingsheng Liu 

机构地区：[1]Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China [2]University of Chinese Academy of Sciences,Beijing 100049,China [3]Institute of Infectious Diseases,Beijing Key Laboratory of Emerging Infectious Diseases,Beijing Ditan Hospital,Capital Medical University,Beijing 100015,China [4]Beijing Institute of Infectious Diseases,Beijing 100015,China

出　　处：《Biophysics Reports》2024年第2期102-110,共9页生物物理学报（英文版）

基　　金：supported by the National Key R&D Program of China(2018YFA0800700,2018YFA0800900 and 2023YFA1801100);National Natural Science Foundation of China(32170787,91954108,91857201 and 32100557)。

摘　　要：The lipid droplet(LD)is a conserved organelle that exists in almost all organisms,ranging from bacteria to mammals.Dysfunctions in LDs are linked to a range of human metabolic syndromes.The formation of protein complexes on LDs is crucial for maintaining their function.Investigating how proteins interact on LDs is essential for understanding the role of LDs.We have developed an effective method to uncover protein–protein interactions and protein complexes specifically on LDs.In this method,we conduct co-immunoprecipitation(co-IP)experiments using LD proteins extracted directly from isolated LDs,rather than utilizing proteins from cell lysates.To elaborate,we begin by purifying LDs with high-quality and extracting LD-associated proteins.Subsequently,the co-IP experiment is performed on these LD-associated proteins directly,which would enhance the co-IP experiment specificity of LDassociated proteins.This method enables researchers to directly unveil protein complexes on LDs and gain deeper insights into the functional roles of proteins associated with LDs.

关 键 词：Lipid droplet CO-IMMUNOPRECIPITATION Protein interaction 

分 类 号：Q503[生物学—生物化学]