基于Box-Behnken响应面设计优选中药复方便乃通的提取工艺  

Optimization of the extraction process for Chinese herbal compound Biannaitong based on Box-Behnken response surface design

作　　者：殷倩倩 邓秀平 钟雨虹 李鑫[1,2] 李小英 刘锴 李嘉瑞 付茉莉 刘志东[1,2] 李亦武 YIN Qianqian;DENG Xiuping;ZHONG Yuhong;LI Xin;LI Xiaoying;LIU Kai;LI Jiarui;FU Moli;LIU Zhidong;LI Yiwu(State Key Laboratory of Component Chinese Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China;Modern Traditional Chinese Medicine Discovery and Formulation Technology Engineering Research Center of the Ministry of Education,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China;Wuhan Tongji Modern Pharmaceutical Technology Co.,Ltd.,Wuhan 430056,China)

机构地区：[1]天津中医药大学组分中药国家重点实验室,天津301617 [2]天津中医药大学现代中药发现与制剂技术教育部工程研究中心,天津301617 [3]武汉同济现代医药科技股份有限公司,武汉430056

出　　处：《天津中医药》2024年第6期789-794,共6页Tianjin Journal of Traditional Chinese Medicine

摘　　要：[目的]利用Box-Behnken响应面设计法优化中药复方便乃通(BNT)的提取工艺。[方法]基于单因素实验优选BNT提取工艺的影响因素及其范围,运用Box-Behnken响应面设计法构建三因素三水平实验设计方案,以番泻苷B、番泻苷A及橙皮苷的含量作为响应指标优选最佳提取工艺条件,并进行工艺验证。[结果]中药复方BNT的最佳提取工艺条件为料液比1∶8,回流时间30 min,提取次数2次。[结论]利用Box-Behnken响应面设计法优化中药复方BNT提取工艺方法稳定,可为BNT提取工艺提供参考。[Objective]To optimize the extraction process of Chinese medicine compound Biannaitong(BNT)by Box-Behnken response surface design.[Methods]Based on the single factor test,the influencing factors and range of BNT extraction process were optimized.The three-factor and three-level test design scheme was constructed by Box-Behnken response surface design method.The content of sennoside B,sennoside A and hesperidin was used as the response index to optimize the best extraction process conditions,and the process verification was carried out.[Results]The optimum extraction conditions of Chinese herbal compound BNT were as follows:solid-liquid ratio of 1∶8,reflux time of 30 min,extracting for 2 times.[Conclusion]The Box-Behnken response surface design method was used to optimize the BNT extraction process of traditional Chinese medicine compound.The method is stable and can provide a reference for the BNT extraction process.

关 键 词：响应面设计 提取工艺 HPLC 

分 类 号：R284.2[医药卫生—中药学]