非小细胞肺癌UCH-L1和PD-L1表达的相关性及其临床病理学意义  

Correlation between UCH-L1 and PD-L1 expression in non-small cell lung cancer and their clinicopathological significance

作　　者：薛庆明 魏东山 江洪 项晶晶 王金洁 XUE Qingming;WEI Dongshan;JIANG Hong;XIANG Jingjing;WANG Jinjie(Department of Cardiothoracic Surgery,Affiliated Hangzhou First People's Hospital,School of Medicine,Westlake University,Hangzhou 310006,China;不详)

机构地区：[1]西湖大学医学院附属杭州市第一人民医院心胸外科,310006 [2]西湖大学医学院附属杭州市第一人民医院病理科,310006

出　　处：《浙江医学》2024年第12期1270-1274,I0006,共6页Zhejiang Medical Journal

基　　金：浙江省基础公益研究计划项目(LGF21H160021);浙江省中医药科技计划项目(2023ZL562)。

摘　　要：目的探讨泛素羧基末端水解酶1(UCH-L1)和程序性细胞死亡因子配体1(PD-L1)在非小细胞肺癌(NSCLC)中表达的相关性及其临床病理学意义。方法回顾性选取2018年1月至2019年12月西湖大学医学院附属杭州市第一人民医院存档的105例患者肿瘤组织石蜡标本和12例患者液氮保存的新鲜肿瘤组织和配对癌旁组织标本。采用Western blot法检测12例NSCLC新鲜组织和配对癌旁组织中UCH-L1和PD-L1蛋白表达水平。采用免疫组化En Vision二步法检测105例NSCLC石蜡包埋组织UCH-L1与PD-L1蛋白表达水平。分析NSCLC组织UCH-L1、PD-L1蛋白表达与临床病理特征和预后的关系及两者蛋白表达的相关性。结果12例患者中,9例(除例6、9和12外)NSCLC新鲜组织UCH-L1蛋白表达水平明显高于配对癌旁组织,10例(除例2、11外)PD-L1蛋白表达水平明显低于配对癌旁组织。UCH-L1和PD-L1在NSCLC肿瘤组织中阳性表达率分别为28.6%(30/105)和38.1%(40/105)。NSCLC组织中UCH-L1蛋白表达与患者肿瘤类型、肿瘤直径和TNM分期均有关(均P<0.05),而PD-L1蛋白表达仅与患者肿瘤类型有关(P=0.001)。UCH-L1阳性表达的NSCLC患者累积无进展生存率低于UCH-L1阴性表达患者(P<0.001),而PD-L1蛋白阳性与阴性表达患者累积无进展生存率比较差异无统计学意义(P=0.055)。Pearson相关分析显示,NSCLC组织UCH-L1与PD-L1蛋白表达呈正相关(r=0.329,P=0.001)。结论UCH-L1在NSCLC发生、发展中起着癌基因的作用,UCH-L1与PD-L1的表达呈正相关,两者可能具有协同作用,共同参与NSCLC的发生、发展。Objective To investigate the correlation between ubiquitin carboxy terminal hydrolase 1(UCH-L1)and programmed cell death ligand 1(PD-L1)expression and their clinicopathological significance in non-small cell lung cancer(NSCLC).Methods A total of 105 paraffin embedded NSCLC samples and 12 paired NSCLC tissue and adjacent normal tissue samples were retrospectively collected from Affiliated Hangzhou First People's Hospital,School of Medicine,Westlake University from January 2018 to December 2019.The expression of UCH-L1 and PD-L1 in 12 fresh paired NSCLC and adjacent normal tissue samples and in 105 paraffin embedded NSCLC samples was detected by Western blot and En Vision two-step immunohistochemistry,respectively.The association between UCH-L1 and PD-L1 protein expression,clinicopathological features,and prognosis of NSCLC tissues,as well as the correlation between the two protein expression were analyzed.Results The results showed that among the 12 patients,the level of UCH-L1 protein expression in fresh NSCLC tissues was significantly higher than that in adjacent normal tissues in 9 cases(except cases 6,9,and 12),while the level ofPD-L1 protein expression was significantly lower than that in adjacent normal tissues in 10 cases(except cases 2 and 11).The positive rate of UCH-L1 and PD-L1 in 105 cases of NSCLC was 28.6%(30/105)and 38.1%(40/105),respectively.UCHL1 expression was associated with tumor type,tumor size and TNM stage(all P<0.05),while PD-L1 expression was just associated with tumor type(P=0.001).The NSCLC patients with positive UCH-L1 expression had a worse progression-free survival(PFS)rate than those with negative expression(P<0.001),while there was no significant difference in PFS rate between patients with positive and negative PD-L1 expression(P=0.055).Pearson correlation analysis revealed that UCH-L1 expression was positively associated with PD-L1 expression(r=0.329,P=0.001).Conclusion UCH-L1 acts as a tumor gene in the occurrence and development of NSCLC.The expression of UCH-L1 and PD-L1 is pos

关 键 词：非小细胞肺癌 泛素羧基末端水解酶1 程序性细胞死亡因子配体1 

分 类 号：R734.2[医药卫生—肿瘤]