CYP2C9对肝细胞癌的迁移、侵袭和增殖的影响  

Effect of CYP2C9 on the migration,invasion and proliferation of hepatocellular carcinoma cells

作　　者：张拓 李达军 王海涛[1] 张鹏[1] ZHANG Tuo;LI Dajun;WANG Haitao;ZHANG Peng(Wuhan University TaiKang Medical School(School of Basic Medical Sciences),Wuhan 430071,China)

机构地区：[1]武汉大学泰康医学院(基础医学院),武汉430071

出　　处：《医学新知》2024年第6期674-681,共8页New Medicine

基　　金：国家自然科学基金面上项目(81970011)。

摘　　要：目的探究CYP2C9是否参与肝细胞癌(hepatocellular carcinoma,HCC)的发生发展及其对肝癌细胞迁移、侵袭和增殖的影响。方法从TCGA数据库中提取HCC的转录组数据,分析CYP2C9在HCC中的表达和预后相关性;构建HepG2和MHCC97H的CYP2C9稳定过表达细胞系,通过Transwell迁移实验、Transwell侵袭实验和Western Blot实验分别检测过表达CYP2C9对肝癌细胞系迁移、侵袭能力以及上皮间质转化(epithelial-mesenchymal transition,EMT)过程的影响;通过CCK-8实验、平板克隆形成实验和Western Blot实验检测过表达CYP2C9对肝癌细胞系增殖能力的影响。结果通过生信分析发现,CYP2C9在HCC中的表达显著下调,且CYP2C9的表达与HCC患者的临床预后呈正相关;细胞及分子实验结果显示,过表达CYP2C9显著抑制HepG2细胞和MHCC97H细胞的迁移、侵袭能力和EMT过程,而对细胞的增殖能力没有显著影响。结论CYP2C9可能通过抑制肝癌细胞的迁移、侵袭以及EMT过程,进而影响HCC的进展。Objective To explore whether CYP2C9 is involved in the occurrence and development of hepatocellular carcinoma(HCC)and its function in the migration,invasion and proliferation of HCC cells.Methods The transcriptome data of HCC were retrieved from the TCGA database to analyze the expression of CYP2C9 and role of CYP2C9 in the prognosis of HCC;Constructing stable CYP2C9 overexpression cell lines of HepG2 and MHCC97H,detecting the effects of CYP2C9 overexpression on the migration,invasion and epithelial mesenchymal transition(EMT)process of HCC cells through Transwell migration assay,Transwell invasion assay,and Western Blot assay,respectively;The effect of CYP2C9 overexpression on the proliferation ability of HCC cells was detected through CCK-8 assay,colony formation assay,and Western Blot assay.Results Bioinformatics analysis showed that the expression of CYP2C9 was significantly downregulated in HCC,and the expression of CYP2C9 was positively correlated with the clinical prognosis of HCC patients;The results of cellular and molecular experiments showed that CYP2C9 overexpression significantly inhibited the migration,invasion and EMT process of HepG2 and MHCC97H cells,whereas had no significant effect on cell proliferation.Conclusion CYP2C9 may affect the progression of HCC by inhibiting the migration,invasion,and EMT processes of HCC cells.

关 键 词：CYP2C9 肝细胞癌 迁移 侵袭 增殖 

分 类 号：R73[医药卫生—肿瘤]