三叶片抑制金黄色葡萄球菌和大肠埃希菌作用的关键组分挖掘  

Key antimicrobial components of Sanye tablets against Staphylococcus aureus and Escherichia coli

作　　者：米卓鑫 齐育麟 李玉红[1,2] 李琳[1,2] 张鹏[1,2,3] 赵鑫[1,2] MI Zhuo-xin;QI Yu-lin;LI Yu-hong;LI Lin;ZHANG Peng;ZHAO Xin(State Key Laboratory of Component-based Chinese Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin 301617;Key Laboratory of Pharmacology of Traditional Chinese Medicine Formulae,Ministry of Education,Tianjin University of Traditional Chinese Medicine,Tianjin 301617;Haihe Laboratory of Modern Chinese Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin 301617)

机构地区：[1]天津中医药大学组分中药国家重点实验室,天津301617 [2]天津中医药大学方剂学教育部重点实验室,天津301617 [3]天津中医药大学现代中医药海河实验室,天津301617

出　　处：《中南药学》2024年第6期1537-1543,共7页Central South Pharmacy

基　　金：国家自然科学基金项目(No.81830112);国家自然科学基金项目(No.81973792);海河一号(中药组分高通量制备平台)的研制(No.22HHZYSS00007)。

摘　　要：目的 挖掘三叶片发挥抑制金黄色葡萄球菌(Staphylococcus aureus,S.aureus)和大肠埃希菌(Escherichia coli,E.coli)作用的关键组分。方法 采用16S rRNA测序技术,在高脂饮食小鼠模型上评价三叶片对肠道菌群的调节作用及对肠道中致病菌S.aureus、E.coli的抑制作用。采用牛津杯法测定三叶片、不同药物组合及其单味药对S.aureus和E.coli的体外抑菌活性,挖掘三叶片中发挥抑菌作用的关键组分,并采用琼脂稀释法研究三叶片及其关键组分对两种致病菌株的最小抑菌浓度(MIC)。结果 菌群测序结果显示,三叶片可以上调高脂饮食小鼠肠道菌群物种丰富度和多样性,降低菌群中包括S.aureus、E.coli等致病菌的丰度。体外抑菌结果显示,三叶片、丹参赤芍组合以及丹参、赤芍组分对上述细菌均有显著的抑制作用。其中三叶片对S.aureus的MIC为0.781 25 mg·mL^(-1),对E.coli的MIC为3.125 mg·mL^(-1)。丹参赤芍组合对S.aureus的MIC为0.25 mg·mL^(-1),对E.coli的MIC为3.125 mg·mL^(-1)。三叶片中丹参组分可显著抑制S.aureus,其MIC为0.125 mg·mL^(-1);赤芍组分对E.coli的抑制作用优于其他组分,其MIC为3.125 mg·mL^(-1)。结论 三叶片能够抑制常见的肠道致病菌如S.aureus和E.coli,该方中丹参为抑制S.aureus的关键组分,赤芍为抑制E.coli的关键组分。Objective To determine the key components of Sanye tablets(SYT)that exert antimicrobial effect on Staphylococcus aureus(S.aureus)and Escherichia coli(E.coli).Methods The regulatory effects on the gut microbiota of SYT and its antimicrobial activity against S.aureus and E.coli were evaluated in high-fat diet mice model by 16S rRNA sequencing.The antimicrobial activity of total formula,different component-mixtures,and single component of SYT against S.aureus and E.coli was investigated by oxford cup method in vitro to determine the key antimicrobial components in SYT.The minimum inhibitory concentration(MIC)of SYT and its key components against these two pathogenic strains were determined with agar dilution method.Results Sequencing results showed that SYT improved the species richness and diversity,and reduced the abundance of pathogenic bacteria,including S.aureus and E.coli in the gut microbiota of high-fat diet mice.The in vitro antimicrobial assay indicated that the SYT,Salviae Miltiorrhizae Radix et Rhizoma(SMR)-Radix Paeoniae Rubra(RPR)co-components,as well as the respective component of SMR and RPR,had significant inhibitory effect on these two pathogenic bacteria.The MIC of SYT aganist S.aureus and E.coli were 0.78125 and 3.125 mg·mL^(-1),respectively.The combination of SMR and RPR showed MIC of 0.25 mg·mL^(-1) against S.aureus and 3.125 mg·mL^(-1) against E.coli.The SMR showed MIC of 0.125 mg·mL^(-1) against S.aureus.The MIC of the RPR was 3.125 mg·mL^(-1) against E.coli,lower than that of other single components.Conclusion SYT shows inhibitory effect on the common intestinal pathogens such as S.aureus and E.coli.SMR is the key component of SYT against S.aureus,and RPR is the key component against E.coli.

关 键 词：三叶片 关键组分 金黄色葡萄球菌 大肠埃希菌 抑菌活性 

分 类 号：R285[医药卫生—中药学]