高表达TOP2A抑制肝癌细胞凋亡及机制  

The Impact and Molecular Mechanism of TOP2A on the Biological Behavior of Liver Cancer Cells

作　　者：候万华 闫佳 王海生 红梅 乔一芸 武瑞兵 HOU Wanhua;YAN Jia;WANG Haisheng;Hongmei;QIAO Yiyun;WU Ruibing(School of Basic Medical Sciences,Inner Mongolia Medical University,Hohhot 010110 China;Peking University Cancer Hospital and Medical Center,Hohhot 010020 China)

机构地区：[1]内蒙古医科大学基础医学院,呼和浩特010110 [2]北京大学肿瘤医院内蒙古医院,呼和浩特010020

出　　处：《内蒙古医学杂志》2024年第3期269-275,共7页Inner Mongolia Medical Journal

基　　金：内蒙古自治区高等学校创新团队发展计划项目(编号:RZ2200000306)。

摘　　要：目的研究TOP2A对肝癌细胞生物学行为的影响及分子机制。方法构建过表达TOP2A细胞株,用RT-qPCR和Western Blot的方法检验载体构建效果,用CCK-8和克隆形成实验检测过表达TOP2A后的HepG2细胞增殖和克隆形成能力的变化,流式细胞术检测细胞凋亡;生物信息学分析与TOP2A相关的蛋白,RT-qPCR和Western Blot检测过表达TOP2A后p53的mRNA水平和蛋白水平变化,Western Blot检测凋亡相关蛋白Casepasse9及Casepasse3表达。结果过表达TOP2A后细胞株的TOP2A表达水平显著增高,差异具有统计学意义(P<0.05);过表达TOP2A后显著促进了HepG2细胞的增殖、迁移和克隆形成能力,减少了细胞的凋亡,差异均具有统计学意义(P<0.05);生物信息学分析TOP2A与p53相互作用密切;过表达TOP2A后显著抑制了p53 mRNA的表达,差异具有统计学意义(P<0.05);过表达TOP2A后显著抑制了p53、Casepasse9和Casepasse3蛋白的表达,差异均具有统计学意义(P<0.05)。结论高表达的TOP2A会阻滞p53介导的信号通路,减少Casepasse9和Casepasse3介导的凋亡并促进肝癌细胞增殖、迁移和克隆形成。Objective To investigate the effects and molecular mechanisms of TOP2A on the biological behavior of liver cancer cells.Methods TOP2A-overexpressing cell lines wereestablished and examined using RT-qPCR and Western blot.After overexpression of TOP2A,the proliferation and clonogenoc ability of HepG2 cells were assessed using CCK-8 and clonogenic assays,while flow cytometry was employed to detect cell apoptosis.Bioinformatics analysis was performed to identify TOP2A-related proteins,and changes in mRNA and protein levels of p53 were detected using RT-qPCR and Western blot afteroverexpression of TOP2A.Expression of apoptosis-related proteins,including Caspase9 and Caspase3,were also detected using Western blot.Results After overexpression of TOP2A,the expression level of TOP2A in cell lines increased significantly(P<0.05).Overexpression of TOP2A significantly increased the proliferation,migration,and clonogenic ability of HepG2 cells,and reduced the cell apoptosis(P<0.05).Bioinformatics analysis showed that there was a close interaction between TOP2A and p53.Overexpression of TOP2A significantly inhibited the expression of p53 mRNA(P<0.05).At the same time,overexpression of TOP2A significantly inhibited the expression of p53,Casepasse9,and Casepasse3 proteins(P<0.05).Conclusion Overexpression of TOP2A may inhibit the p53 mediated signaling pathway,reduce the apoptosis mediated by Caspase9 and Caspase3,and promote the proliferation,migration,and clonal formation of liver cancer cells.

关 键 词：TOP2A P53 生物学行为 凋亡 肝癌 

分 类 号：R735.7[医药卫生—肿瘤]