Wild soybean(Glycine soja)transcription factor GsWRKY40 plays positive roles in plant salt tolerance  

作　　者：Minglong Li Man Xue Huiying Ma Peng Feng Tong Chen Xiaohuan Sun Qiang Li Xiaodong Ding Shuzhen Zhang Jialei Xiao 

机构地区：[1]Key Laboratory of Agricultural Biological Functional Genes,Northeast Agricultural University,Harbin 150030,Heilongjiang,China [2]Key Laboratory of Soybean Biology of Chinese Education Ministry,Northeast Agricultural University,Harbin 150030,Heilongjiang,China [3]Department of Plant Science,School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China [4]College of Life Science,Leshan Normal University,Leshan 614000,Sichuan,China [5]Mudanjiang Branch of Heilongjiang Academy of Agricultural Sciences,Mudanjiang 157401,Heilongjiang,China

出　　处：《The Crop Journal》2024年第3期766-775,共10页作物学报（英文版）

基　　金：financially supported by the National Key Research and Development Program of China(2021YFD120110402);the National Natural Science Foundation of China(32272048,32272017);the Natural Science Foundation of Heilongjiang Province(LH2022C019)。

摘　　要：Wild soybean(Glycine soja),a relative of cultivated soybean,shows high adaptability to adverse environmental conditions.We identified and characterized a wild soybean transcription factor gene,GsWRKY40,that promotes plant salt stress.GsWRKY40 was highly expressed in wild soybean roots and was up-regulated by salt treatment.GsWRKY40 was localized in nucleus and demonstrated DNA-binding activities but without transcriptional activation.Mutation and overexpression of GsWRKY40 altered salt tolerance of Arabidopsis plants.To understand the molecular mechanism of GsWRKY40 in regulating plant salt resistance,we screened a cDNA library and identified a GsWRKY40 interacting protein GsbHLH92 by using yeast two-hybrid approach.The physical interaction of GsWRKY40 and GsbHLH92 was confirmed by co-immunoprecipitation(co-IP),GST pull-down,and bimolecular fluorescence complementation(BiFC)techniques.Intriguingly,co-overexpression of GsWRKY40 and GsbHLH92 resulted in higher salt tolerance and lower ROS levels than overexpression of GsWRKY40 or GsbHLH92 in composite soybean plants,suggesting that GsWRKY40 and GsbHLH92 may synergistically regulate plant salt resistance through inhibiting ROS production.qRT-PCR data indicated that the expression level of GmSPOD1 gene encoding peroxidase was cooperatively regulated by GsWRKY40 and GsbHLH92,which was confirmed by using a dual luciferase report system and yeast one-hybrid experiment.Our study reveals a pathway that GsWRKY40 and GsbHLH92 collaboratively up-regulate plant salt resistance through impeding GmSPOD1 expression and reducing ROS levels,providing a novel perspective on the regulatory mechanisms underlying plant tolerance to abiotic stresses.

关 键 词：Wild soybean Transcription factor Salt stress ROS 

分 类 号：S565.1[农业科学—作物学]