Gene expression profiles in early leaf of rice(Oryza sativa)and foxtail millet(Setaria italica)  

作　　者：Jing Sun Chen Deng Xiuru Dai Haoshu Li Liying Zhang Jingke Wang Hang Zhao Yirong Yang NghiVan Phung Zhiguo Zhang Pinghua Li Xuehui Sun Tiegang Lu 

机构地区：[1]Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China [2]College of Agronomy,Shandong Agricultural University,Tai’an 271018,Shandong,China [3]Qingdao Institute of Bioenergy and Bioprocess Technology,Chinese Academy of Sciences,Qingdao 266101,Shandong,China

出　　处：《The Crop Journal》2024年第3期776-787,共12页作物学报（英文版）

基　　金：supported by the National Key Research and Development Program of China(NKRDP)(2022YFF1001700);the Agricultural Science and Technology Innovation Program(2020YFE0202300);the National Natural Science Foundation of China(31871313)。

摘　　要：Plant anatomy is patterned early during leaf development which suggests studying the spatial–temporal transcriptomes of primordia will help identify critical regulative and functional genes.We successfully isolated the leaf primordia tissues from the C3grass rice and the C4grass foxtail millet by laser capture microdissection(LCM)and studied the gene expression throughout leaf developmental stages.Our data analysis uncovered the conserved expression patterns of certain gene clusters both in rice and foxtail millet during leaf development.We revealed genes and transcription factors involved in vein formation,stomatal development,and suberin accumulation.We identified 79 candidate genes associated with functional regulation of C4anatomy formation.Screening phenotype of the candidate genes revealed that knock-out of a putative polar auxin transport related gene NAL1 resulted significantly reduced veinal space in rice leaf.Our present work provides a foundation for future analyses of genes with novel functions in grasses and their role in leaf development,in particular the role in leaves with a contrasting C3vs.C4biosynthetic pathway.

关 键 词：RICE Foxtail millet Leaf development RNA-SEQ LCM 

分 类 号：S511[农业科学—作物学] S515